FUSIP1 explained

FUS-interacting serine-arginine-rich protein 1 is a protein that in humans is encoded by the SFRS13A gene.^{[1] [2] [3]}

Function

This gene product is a member of the serine-arginine (SR) family of proteins, which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS and abrogates the influence of TLS on E1A pre-mRNA splicing. Alternative splicing of this gene results in at least two transcript variants encoding different isoforms. In addition, transcript variants utilizing alternative polyA sites exist.

Interactions

FUSIP1 has been shown to interact with FUS.^[4]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA . A "double adaptor" method for improved shotgun library construction . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 .
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA . Large-scale concatenation cDNA sequencing . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 139146 . 10.1101/gr.7.4.353 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Yang L, Embree LJ, Hickstein DD . TLS-ERG leukemia fusion protein inhibits RNA splicing mediated by serine-arginine proteins . Mol. Cell. Biol. . 20 . 10 . 3345–54 . 2000 . 10779324 . 85627 . 10.1128/MCB.20.10.3345-3354.2000 .
• Cowper AE, Cáceres JF, Mayeda A, Screaton GR . Serine-arginine (SR) protein-like factors that antagonize authentic SR proteins and regulate alternative splicing . J. Biol. Chem. . 276 . 52 . 48908–14 . 2001 . 11684676 . 10.1074/jbc.M103967200 . free .
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI . Directed proteomic analysis of the human nucleolus . Curr. Biol. . 12 . 1 . 1–11 . 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . 14132033 . free . 2002CBio...12....1A .
• Shin C, Manley JL . The SR protein SRp38 represses splicing in M phase cells . Cell . 111 . 3 . 407–17 . 2002 . 12419250 . 10.1016/S0092-8674(02)01038-3 . 13215962 . free .
• Li J, Hawkins IC, Harvey CD, Jennings JL, Link AJ, Patton JG . Regulation of alternative splicing by SRrp86 and its interacting proteins . Mol. Cell. Biol. . 23 . 21 . 7437–47 . 2003 . 14559993 . 207616 . 10.1128/MCB.23.21.7437-7447.2003 .
• Shin C, Feng Y, Manley JL . Dephosphorylated SRp38 acts as a splicing repressor in response to heat shock . Nature . 427 . 6974 . 553–8 . 2004 . 14765198 . 10.1038/nature02288 . 2004Natur.427..553S . 4423246 .
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 514446 . 10.1073/pnas.0404720101 . 2004PNAS..10112130B . free .
• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization . Curr. Biol. . 14 . 16 . 1436–50 . 2004 . 15324660 . 10.1016/j.cub.2004.07.051 . 2371325 . free . 2004CBio...14.1436J .
• Benzinger A, Muster N, Koch HB, Yates JR, Hermeking H . Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer . Mol. Cell. Proteomics . 4 . 6 . 785–95 . 2005 . 15778465 . 10.1074/mcp.M500021-MCP200 . free .
• Shin C, Kleiman FE, Manley JL . Multiple properties of the splicing repressor SRp38 distinguish it from typical SR proteins . Mol. Cell. Biol. . 25 . 18 . 8334–43 . 2005 . 16135820 . 1234314 . 10.1128/MCB.25.18.8334-8343.2005 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .

Notes and References

1. Yang L, Embree LJ, Tsai S, Hickstein DD . Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing . J. Biol. Chem. . 273 . 43 . 27761–4 . November 1998 . 9774382 . 10.1074/jbc.273.43.27761 . free .
2. Clinton JM, Chansky HA, Odell DD, Zielinska-Kwiatkowska A, Hickstein DD, Yang L . Characterization and expression of the human gene encoding two translocation liposarcoma protein-associated serine-arginine (TASR) proteins . Gene . 284 . 1–2 . 141–7 . March 2002 . 11891055 . 10.1016/S0378-1119(02)00382-7 .
3. Web site: Entrez Gene: FUSIP1 FUS interacting protein (serine/arginine-rich) 1.
4. Yang L, Embree LJ, Hickstein DD . TLS-ERG leukemia fusion protein inhibits RNA splicing mediated by serine-arginine proteins . Mol. Cell. Biol. . 20 . 10 . 3345–54 . May 2000 . 10779324 . 85627 . 10.1128/MCB.20.10.3345-3354.2000 .