FMN reductase (NADPH) explained

FMN reductase (NADPH)
Ec Number:1.5.1.38

FMN reductase (NADPH) (FRP, flavin reductase P, SsuE) is an enzyme with systematic name FMNH2:NADP+ oxidoreductase.[1] [2] [3] [4] [5] [6] [7] [8] This enzyme catalyses the following chemical reaction:

FMNH2 + NADP+

\rightleftharpoons

FMN + NADPH + H+

The enzymes from bioluminescent bacteria contain FMN.

Notes and References

  1. Gerlo E, Charlier J . Identification of NADH-specific and NADPH-specific FMN reductases in Beneckea harveyi . European Journal of Biochemistry . 57 . 2 . 461–7 . September 1975 . 1175652 . 10.1111/j.1432-1033.1975.tb02321.x . free .
  2. Jablonski E, DeLuca M . Purification and properties of the NADH and NADPH specific FMN oxidoreductases from Beneckea harveyi . Biochemistry . 16 . 13 . 2932–6 . June 1977 . 880288 . 10.1021/bi00632a020 .
  3. Jablonski E, DeLuca M . Studies of the control of luminescence in Beneckea harveyi: properties of the NADH and NADPH:FMN oxidoreductases . Biochemistry . 17 . 4 . 672–8 . February 1978 . 23827 . 10.1021/bi00597a018 .
  4. Lei B, Liu M, Huang S, Tu SC . Vibrio harveyi NADPH-flavin oxidoreductase: cloning, sequencing and overexpression of the gene and purification and characterization of the cloned enzyme . Journal of Bacteriology . 176 . 12 . 3552–8 . June 1994 . 8206832 . 205543 . 10.1128/jb.176.12.3552-3558.1994 .
  5. Tanner JJ, Lei B, Tu SC, Krause KL . Flavin reductase P: structure of a dimeric enzyme that reduces flavin . Biochemistry . 35 . 42 . 13531–9 . October 1996 . 8885832 . 10.1021/bi961400v .
  6. Liu M, Lei B, Ding Q, Lee JC, Tu SC . Vibrio harveyi NADPH:FMN oxidoreductase: preparation and characterization of the apoenzyme and monomer-dimer equilibrium . Archives of Biochemistry and Biophysics . 337 . 1 . 89–95 . January 1997 . 8990272 . 10.1006/abbi.1996.9746 .
  7. Lei B, Tu SC . Mechanism of reduced flavin transfer from Vibrio harveyi NADPH-FMN oxidoreductase to luciferase . Biochemistry . 37 . 41 . 14623–9 . October 1998 . 9772191 . 10.1021/bi981841+ .
  8. Eichhorn E, van der Ploeg JR, Leisinger T . Characterization of a two-component alkanesulfonate monooxygenase from Escherichia coli . The Journal of Biological Chemistry . 274 . 38 . 26639–46 . September 1999 . 10480865 . 10.1074/jbc.274.38.26639 . free .

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