40S ribosomal protein S30 explained

40S ribosomal protein S30 is a protein that in humans is encoded by the FAU gene.^{[1] [2]}

Function

This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein FUBI at the N-terminus and ribosomal protein S30 at the C-terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.^[2]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins. . Biochem. Cell Biol. . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
• Kas K, Michiels L, Merregaert J . Genomic structure and expression of the human fau gene: encoding the ribosomal protein S30 fused to a ubiquitin-like protein . Biochem. Biophys. Res. Commun. . 187 . 2 . 927–33 . 1992 . 1326960 . 10.1016/0006-291X(92)91286-Y .
• Kato S, Sekine S, Oh SW . Construction of a human full-length cDNA bank . Gene . 150 . 2 . 243–50 . 1995 . 7821789 . 10.1016/0378-1119(94)90433-2 . etal.
• Michiels L, Van der Rauwelaert E, Van Hasselt F . fau cDNA encodes a ubiquitin-like-S30 fusion protein and is expressed as an antisense sequence in the Finkel-Biskis-Reilly murine sarcoma virus . Oncogene . 8 . 9 . 2537–46 . 1993 . 8395683 . etal.
• Vladimirov SN, Ivanov AV, Karpova GG . Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry . Eur. J. Biochem. . 239 . 1 . 144–9 . 1996 . 8706699 . 10.1111/j.1432-1033.1996.0144u.x . etal. free .
• Kenmochi N, Kawaguchi T, Rozen S . A map of 75 human ribosomal protein genes . Genome Res. . 8 . 5 . 509–23 . 1998 . 9582194 . 10.1101/gr.8.5.509. etal. free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Rossman TG, Visalli MA, Komissarova EV . fau and its ubiquitin-like domain (FUBI) transforms human osteogenic sarcoma (HOS) cells to anchorage-independence . Oncogene . 22 . 12 . 1817–21 . 2003 . 12660817 . 10.1038/sj.onc.1206283. 21256130 .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Suzuki Y, Yamashita R, Shirota M . Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions . Genome Res. . 14 . 9 . 1711–8 . 2004 . 15342556 . 10.1101/gr.2435604 . 515316 . etal.
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Mourtada-Maarabouni M, Kirkham L, Farzaneh F, Williams GT . Regulation of apoptosis by fau revealed by functional expression cloning and antisense expression . Oncogene . 23 . 58 . 9419–26 . 2005 . 15543234 . 10.1038/sj.onc.1208048 . 38364471 .
• Andersen JS, Lam YW, Leung AK . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 . etal.
• Yu Y, Ji H, Doudna JA, Leary JA . Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes . Protein Sci. . 14 . 6 . 1438–46 . 2005 . 15883184 . 10.1110/ps.041293005 . 2253395 .

Notes and References

1. Kas K, Schoenmakers E, van de Ven W, Weber G, Nordenskjold M, Michiels L, Merregaert J, Larsson C . Assignment of the human FAU gene to a subregion of chromosome 11q13 . Genomics . 17 . 2 . 387–92 . Oct 1993 . 8406491 . 10.1006/geno.1993.1337 .
2. Web site: Entrez Gene: FAU Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived); ribosomal protein S30.