FABP5 explained

Fatty acid-binding protein, epidermal is a protein that in humans is encoded by the FABP5 gene.^{[1] [2]}

Function

This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.^[2]

The phytocannabinoids (THC and CBD) inhibit endocannabinoid anandamide (AEA) uptake by targeting FABP5, and competition for FABPs may in part or wholly explain the increased circulating levels of endocannabinoids reported after consumption of cannabinoids.^[3] Results show that cannabinoids inhibit keratinocyte proliferation, and therefore support a potential role for cannabinoids in the treatment of psoriasis.^[4]

Interactions

FABP5 has been shown to interact with S100A7.^{[5] [6]}

Further reading

• Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J . Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes . Electrophoresis . 13 . 12 . 960–9 . December 1992 . 1286667 . 10.1002/elps.11501301199 . 41855774 .
• Siegenthaler G, Hotz R, Chatellard-Gruaz D, Didierjean L, Hellman U, Saurat JH . Purification and characterization of the human epidermal fatty acid-binding protein: localization during epidermal cell differentiation in vivo and in vitro . The Biochemical Journal . 302 (Pt 2) . 2 . 363–71 . September 1994 . 8092987 . 1137237 . 10.1042/bj3020363.
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . January 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Siegenthaler G, Hotz R, Chatellard-Gruaz D, Jaconi S, Saurat JH . Characterization and expression of a novel human fatty acid-binding protein: the epidermal type (E-FABP) . Biochemical and Biophysical Research Communications . 190 . 2 . 482–7 . January 1993 . 8427590 . 10.1006/bbrc.1993.1073 .
• Masouyé I, Saurat JH, Siegenthaler G . Epidermal fatty-acid-binding protein in psoriasis, basal and squamous cell carcinomas: an immunohistological study . Dermatology . 192 . 3 . 208–13 . 1996 . 8726632 . 10.1159/000246367 .
• Jaworski C, Wistow G . LP2, a differentiation-associated lipid-binding protein expressed in bovine lens . The Biochemical Journal . 320 (Pt 1) . 1 . 49–54 . November 1996 . 8947466 . 1217896 . 10.1042/bj3200049.
• Masouyé I, Hagens G, Van Kuppevelt TH, Madsen P, Saurat JH, Veerkamp JH, Pepper MS, Siegenthaler G . Endothelial cells of the human microvasculature express epidermal fatty acid-binding protein . Circulation Research . 81 . 3 . 297–303 . September 1997 . 9285630 . 10.1161/01.res.81.3.297 . 2066/25768 . 21737640 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . October 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Watanabe R, Fujii H, Yamamoto A, Hashimoto T, Kameda K, Ito M, Ono T . Immunohistochemical distribution of cutaneous fatty acid-binding protein in human skin . Journal of Dermatological Science . 16 . 1 . 17–22 . November 1997 . 9438903 . 10.1016/S0923-1811(97)00615-4 .
• Hagens G, Masouyé I, Augsburger E, Hotz R, Saurat JH, Siegenthaler G . Calcium-binding protein S100A7 and epidermal-type fatty acid-binding protein are associated in the cytosol of human keratinocytes . The Biochemical Journal . 339 (Pt 2) . 2 . 419–27 . April 1999 . 10191275 . 1220173 . 10.1042/0264-6021:3390419 .
• Hagens G, Roulin K, Hotz R, Saurat JH, Hellman U, Siegenthaler G . Probable interaction between S100A7 and E-FABP in the cytosol of human keratinocytes from psoriatic scales . Molecular and Cellular Biochemistry . 192 . 1–2 . 123–8 . February 1999 . 10331666 . 10.1023/A:1006894909694 . 24171894 .
• Hohoff C, Börchers T, Rüstow B, Spener F, van Tilbeurgh H . Expression, purification, and crystal structure determination of recombinant human epidermal-type fatty acid binding protein . Biochemistry . 38 . 38 . 12229–39 . September 1999 . 10493790 . 10.1021/bi990305u .
• O'Shaughnessy RF, Seery JP, Celis JE, Frischauf A, Watt FM . PA-FABP, a novel marker of human epidermal transit amplifying cells revealed by 2D protein gel electrophoresis and cDNA array hybridisation . FEBS Letters . 486 . 2 . 149–54 . December 2000 . 11113456 . 10.1016/S0014-5793(00)02252-3 . 23327194 . 2000FEBSL.486..149O .
• Gutiérrez-González LH, Ludwig C, Hohoff C, Rademacher M, Hanhoff T, Rüterjans H, Spener F, Lücke C . Solution structure and backbone dynamics of human epidermal-type fatty acid-binding protein (E-FABP) . The Biochemical Journal . 364 . Pt 3 . 725–37 . June 2002 . 12049637 . 1222622 . 10.1042/BJ20020039 .
• Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J . Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides . Nature Biotechnology . 21 . 5 . 566–9 . May 2003 . 12665801 . 10.1038/nbt810 . 23783563 .
• Ruse M, Broome AM, Eckert RL . S100A7 (psoriasin) interacts with epidermal fatty acid binding protein and localizes in focal adhesion-like structures in cultured keratinocytes . The Journal of Investigative Dermatology . 121 . 1 . 132–41 . July 2003 . 12839573 . 10.1046/j.1523-1747.2003.12309.x . free .
• Ha MK, Chung KY, Lee JH, Bang D, Park YK, Lee KH . Expression of psoriasis-associated fatty acid-binding protein in senescent human dermal microvascular endothelial cells . Experimental Dermatology . 13 . 9 . 543–50 . September 2004 . 15335354 . 10.1111/j.0906-6705.2004.00196.x . 22923459 .

Notes and References

1. Madsen P, Rasmussen HH, Leffers H, Honoré B, Celis JE . Molecular cloning and expression of a novel keratinocyte protein (psoriasis-associated fatty acid-binding protein [PA-FABP]) that is highly up-regulated in psoriatic skin and that shares similarity to fatty acid-binding proteins . The Journal of Investigative Dermatology . 99 . 3 . 299–305 . September 1992 . 1512466 . 10.1111/1523-1747.ep12616641 .
2. Web site: Entrez Gene: FABP5 fatty acid binding protein 5 (psoriasis-associated).
3. Elmes MW, Kaczocha M, Berger WT, Leung K, Ralph BP, Wang L, Sweeney JM, Miyauchi JT, Tsirka SE, Ojima I, Deutsch DG . Fatty acid-binding proteins (FABPs) are intracellular carriers for Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) . The Journal of Biological Chemistry . 290 . 14 . 8711–21 . April 2015 . 25666611 . 4423662 . 10.1074/jbc.M114.618447 . free .
4. Wilkinson JD, Williamson EM . Cannabinoids inhibit human keratinocyte proliferation through a non-CB1/CB2 mechanism and have a potential therapeutic value in the treatment of psoriasis . en . Journal of Dermatological Science . 45 . 2 . 87–92 . February 2007 . 17157480 . 10.1016/j.jdermsci.2006.10.009 .
5. Ruse M, Broome AM, Eckert RL . S100A7 (psoriasin) interacts with epidermal fatty acid binding protein and localizes in focal adhesion-like structures in cultured keratinocytes . The Journal of Investigative Dermatology . 121 . 1 . 132–41 . July 2003 . 12839573 . 10.1046/j.1523-1747.2003.12309.x . free .
6. Hagens G, Roulin K, Hotz R, Saurat JH, Hellman U, Siegenthaler G . Probable interaction between S100A7 and E-FABP in the cytosol of human keratinocytes from psoriatic scales . Molecular and Cellular Biochemistry . 192 . 1–2 . 123–8 . February 1999 . 10331666 . 10.1023/A:1006894909694 . 24171894 .