Expression cassette explained

An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell.^[1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette can easily be altered to make different proteins.^[2]

An expression cassette is composed of one or more genes and the sequences controlling their expression.^[3] An expression cassette comprises three components: a promoter sequence, an open reading frame, and a 3' untranslated region that, in eukaryotes, usually contains a polyadenylation site.^[4]

Different expression cassettes can be transfected into different organisms including bacteria, yeast, plants, and mammalian cells as long as the correct regulatory sequences are used.^{[5] [6]}

See also

• Expression vector
• Gene cassette

Notes and References

1. Promoters and Control Elements: Designing Expression Cassettes for Gene Therapy. Current Gene Therapy. 2004. 4. 89–113. E.D. . Papadakis. 4. 10.2174/1566523044578077. 15032617. etal. 10.1.1.494.3472.
2. The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization. RNA . 1997. 3. 1. 75–88. Edouard . Bertrand. etal. 1369464. 8990401.
3. Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae. Claudia E . Vickers. Microbial Cell Factories. December 2013. 12. 96. 96. 10.1186/1475-2859-12-96. 24161108 . 4231455 . free .
4. Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy. Steven James . Gray. etal. Discov Med . January 2015 . 19. 102. 49–57. 1539-6509 . 4505817 . 25636961.
5. Book: K. . Azhakanandam. etal. 41–52. Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants. 10.1007/978-1-4939-2202-4_2. 2015. Springer. Plant Trait Gene Expression Cassette Design. 978-1-4939-2201-7. 82188623 .
6. Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways. Yu-Hua . Chow. etal. Proceedings of the National Academy of Sciences. 94. 26 . 14695–14700. December 1997. 10.1073/pnas.94.26.14695. 9405675 . 25096. 1997PNAS...9414695C . free .