Exodeoxyribonuclease I Explained

Exodeoxyribonuclease I
Ec Number:	3.1.11.1
Cas Number:	9037-46-1

Exodeoxyribonuclease I (EC 3.1.11.1, Escherichia coli exonuclease I, E. coli exonuclease I, exonuclease I) is an enzyme^{[1] [2] [3]} that catalyses the following chemical reaction:

Exonucleolytic cleavage in the 3′- to 5′-direction to yield nucleoside 5′-phosphates

Preference for single-stranded DNA. The Escherichia coli enzyme hydrolyses glucosylated DNA.Punjabi

Notes and References

1. Blakesley RW, Dodgson JB, Nes IF, Wells RD . Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius . The Journal of Biological Chemistry . 252 . 20 . 7300–6 . October 1977 . 10.1016/S0021-9258(19)66969-3 . free . 71298 .
2. Excision of thymine dimers and other mismatched sequences by DNA polymerases of Escherichia coli . Kelley RB, Atkinson MR, Huberman JA, Kornberg A . Nature . 1969 . 224 . 5218 . 495–501 . 10.1038/224495a0. 1969Natur.224..495K .
3. Lehman IR, Nussbaum AL . The deoxyribonucleases of Escherichia coli. V. On the specificity of exonuclease I (phosphodiesterase) . The Journal of Biological Chemistry . 239 . 2628–36 . August 1964 . 8 . 10.1016/S0021-9258(18)93898-6 . free . 14235546 .