EuroFlow explained

EuroFlow consortium was founded in 2005 as 2U-FP6 funded project and launched in spring 2006. At first, EuroFlow was composed of 18 diagnostic research groups and two SMEs (small/medium enterprises) from eight different European countries with complementary knowledge and skills in the field of flow cytometry and immunophenotyping. During 2012 both SMEs left the project so it obtained full scientific independence. The goal of EuroFlow consortium is to innovate and standardize flow cytometry leading to global improvement and progress in diagnostics of haematological malignancies and individualisation of treatment.

Background

Since the '90s immunophenotyping (staining cells with antibodies conjugated with fluorochromes and detection with flow cytometer) became the preferred method in diagnostics of haematological malignancies. The advantages of this method are speed and simplicity, possibility to measure more than 6 parameters at a time, precise focusing on malignant population and also broad applicability in diagnostics. Because there is a great progress in development of antibodies, fluorochromes and multicolor digital flow cytometers, it became a question of how to interpret cytometric data and how to achieve comparable results between facilities. Even though a consensus of recommendations and guidelines was established, standardization was only partial because there was no regard of different antibody clones, fluorochromes and their optimal combinations or of sample preparation. On that account cytometry is perceived as method highly dependent on level of expertise and with limited reproducibility in multicentric studies.

Goals of Euroflow

These goals were set out in the journal Leukemia in 2012.[1]

Achievements

During passed few years EuroFlow achieved most of its goals. Eight-color panels for diagnoses, and classification and follow-up of haematological malignancies were established.[2] Panels, consisting of screening tube and supplementary characterisation tubes, are based on experiences and knowledge from literature but further optimised and tested in multiple research centers on large collection of samples impeaching on selection of fluorochromes and standardization of instrument settings and SOPs.[3] Antibody clones, fluorochromes and other reagencies from different companies underwent detailed testing and comparison. Simultaneously a new software for analysing of more complex and extensive data files was developed, capable of multidimensional statistical comparison of normal data samples and patient samples.[4] [5] Also new antibody clones against rigorously selected epitopes of proteins involved in chromosomal translocations were developed for detection of most frequent fusion proteins in acute leukemia and chronic myeloid leukemia. Also detection of fusion proteins using immunobead assays was introduced.[6] [7] [8]

External links

Notes and References

  1. 10.1038/leu.2012.121 . EuroFlow: Resetting leukemia and lymphoma immunophenotyping. Basis for companion diagnostics and personalized medicine . 2012 . Van Dongen . J J M . Orfao . A . Leukemia . 26 . 9 . 1899–907 . 22948488 . Euroflow . Consortium . 3437406.
  2. 10.1038/leu.2012.120 . EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes . 2012 . Van Dongen . J J M . Lhermitte . L . Böttcher . S . Almeida . J . Van Der Velden . V H J . Flores-Montero . J . Rawstron . A . Asnafi . V . Lécrevisse . Q . Lucio . P . Mejstrikova . E . Szczepański . T . Kalina . T . De Tute . R . Brüggemann . M . Sedek . L . Cullen . M . Langerak . A W . Mendonça . A . MacIntyre . E . Martin-Ayuso . M . Hrusak . O . Vidriales . M B . Orfao . A . Leukemia . 26 . 9 . 1908–75 . 22552007 . 3437410.
  3. 10.1038/leu.2012.122 . EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols . 2012 . Kalina . T . Flores-Montero . J . Van Der Velden . V H J . Martin-Ayuso . M . Böttcher . S . Ritgen . M . Almeida . J . Lhermitte . L . Asnafi . V . Mendonça . A . De Tute . R . Cullen . M . Sedek . L . Vidriales . M B . Pérez . J J . Te Marvelde . J G . Mejstrikova . E . Hrusak . O . Szczepański . T . Van Dongen . J J M . Orfao . A . Leukemia . 26 . 9 . 1986–2010 . 22948490 . 3437409.
  4. 10.1002/cyto.a.20608 . Generation of flow cytometry data files with a potentially infinite number of dimensions . 2008 . Pedreira . Carlos E. . Costa . Elaine S. . Barrena . Susana . Lecrevisse . Quentin . Almeida . Julia . Van Dongen . Jacques J. M. . Orfao . Alberto . Cytometry Part A . 73A . 9 . 834–46 . 18629843 . Euroflow . Consortium. 12560225 .
  5. 10.1038/leu.2010.160 . Automated pattern-guided principal component analysis vs expert-based immunophenotypic classification of B-cell chronic lymphoproliferative disorders: A step forward in the standardization of clinical immunophenotyping . 2010 . Costa . E S . Pedreira . C E . Barrena . S . Lecrevisse . Q . Flores . J . Quijano . S . Almeida . J . Del Carmen García-Macias . M . Bottcher . S . Van Dongen . J J M . Orfao . A . Leukemia . 24 . 11 . 1927–33 . 20844562 . 3035971.
  6. 10.1016/j.beha.2010.09.010 . Detection of fusion genes at the protein level in leukemia patients via the flow cytometric immunobead assay . 2010 . Dekking . E. . Van Der Velden . V.H.J. . Böttcher . S. . Brüggemann . M. . Sonneveld . E. . Koning-Goedheer . A. . Boeckx . N. . Lucio . P. . Sedek . L. . Szczepański . T. . Kalina . T. . Kovac . M. . Evans . P. . Hoogeveen . P.G. . Flores-Montero . J. . Orfao . A. . Comans-Bitter . W.M. . Staal . F.J.T. . Van Dongen . J.J.M. . Best Practice & Research Clinical Haematology . 23 . 3 . 333–45 . 21123134.
  7. 10.1038/leu.2012.125 . Flow cytometric immunobead assay for fast and easy detection of PML–RARA fusion proteins for the diagnosis of acute promyelocytic leukemia . 2012 . Dekking . E H A . Van Der Velden . V H J . Varro . R . Wai . H . Böttcher . S . Kneba . M . Sonneveld . E . Koning . A . Boeckx . N . Van Poecke . N . Lucio . P . Mendonça . A . Sedek . L . Szczepański . T . Kalina . T . Kanderová . V . Hoogeveen . P . Flores-Montero . J . Chillón . M C . Orfao . A . Almeida . J . Evans . P . Cullen . M . Noordijk . A L . Vermeulen . P M . De Man . M T . Dixon . E P . Comans-Bitter . W M . Van Dongen . J J M . Leukemia . 26 . 9 . 1976–85 . 22948489 . 3437408.
  8. 10.1038/leu.2009.93 . Flow cytometric immunobead assay for the detection of BCR–ABL fusion proteins in leukemia patients . 2009 . Weerkamp . F . Dekking . E . Ng . Y Y . Van Der Velden . V H J . Wai . H . Böttcher . S . Brüggemann . M . Van Der Sluijs . A J . Koning . A . Boeckx . N . Van Poecke . N . Lucio . P . Mendonça . A . Sedek . L . Szczepański . T . Kalina . T . Kovac . M . Hoogeveen . P G . Flores-Montero . J . Orfao . A . MacIntyre . E . Lhermitte . L . Chen . R . Brouwer-De Cock . K A J . Van Der Linden . A . Noordijk . A L . Comans-Bitter . W M . Staal . F J T . Van Dongen . J J M . Leukemia . 23 . 6 . 1106–17 . 19387467 . Euroflow . Consortium.

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