| Official Name: | Eugene, Florida |
| Settlement Type: | Unincorporated community |
| Pushpin Map: | Florida |
| Subdivision Type: | Country |
| Subdivision Name: | United States |
| Subdivision Type1: | State |
| Subdivision Name1: | Florida |
| Subdivision Type2: | County |
| Subdivision Name2: | Dixie |
| Timezone: | Eastern (EST) |
| Utc Offset: | -5 |
| Timezone Dst: | EDT |
| Utc Offset Dst: | -4 |
| Elevation Ft: | 39 |
| Coordinates: | 29.6117°N -83.0878°W |
| Area Code: | 352 |
| Blank Name: | GNIS feature ID |
| Blank Info: | 294758 |
Eugene is an unincorporated area in Dixie County, Florida. The Florida State Road Department rendered Eugene on a 1936 road map.[1] A 1967 state geology report noted Pamlico Dune near Eugene on U.S. Highway 27.[2] Eugene is south of Cross City and North of Old Town, Florida on U.S. Route 98 in Florida. The Nature Coast State Trail also passes by.