Epithelial cell adhesion molecule explained

Epithelial cell adhesion molecule (EpCAM), also known as CD326 among other names, is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia.[1] EpCAM is also involved in cell signaling,[2] migration,[3] proliferation, and differentiation.[4] Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E.[5] Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.[6]

Expression pattern

First discovered in 1979, EpCAM was initially described as a dominant surface antigen on human colon carcinoma.[7] Because of its prevalence on many carcinomas, it has been "discovered" many different times.[8] EpCAM therefore has many aliases the most notable of which include TACSTD1 (tumor-associated calcium signal transducer 1), CD326 (cluster of differentiation 326), and the 17-1A antigen.[9]

EpCAM expression is not limited to human colon carcinomas; in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. However, EpCAM is not found in non-epithelial cells or cancers of non-epithelial origin. EpCAM is expressed on the basolateral membrane of all simple (especially glandular), pseudo-stratified, and transitional epithelia. In contrast, normal squamous stratified epithelia are negative for EpCAM. The level of expression may differ significantly between the individual tissue types. In the gastrointestinal tract, the gastric epithelium expresses very low levels of EpCAM. Expression levels are substantially higher in small intestine, and in colon EpCAM is probably expressed at the highest levels among all epithelial cell types.

EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. In cancer cells, EpCAM is expressed in a dispersed pattern across the cell membrane.[10] However, EpCAM expression in carcinomas is often heterogeneous; some cells in a tumor have more EpCAM than other cells in the same tumor.

Squamous carcinomas often express EpCAM whereas normal squamous cells do not express EpCAM. EpCAM expression differs between different types of renal cell carcinomas, and EpCAM expression increases during development of androgen resistance in prostate cancer.[11] All of this points towards the utility of EpCAM as a diagnostic tool for various cancers.

Structure

Although it is identified as a cell adhesion molecule, EpCAM does not structurally resemble any of the four major families of cell adhesion molecules, namely cadherins, integrins, selectins, and members of the immunoglobulin super-family.

EpCAM is a glycosylated, 30- to 40-kDa type I membrane protein. The sequence of the EpCAM molecule predicts the presence of three potential N-linked glycosylation sites. It is composed of 314 amino acids. EpCAM consists of an extracellular domain (242 amino acids) with epidermal growth factor (EGF)- and thyroglobulin repeat-like domains, a single transmembrane domain (23 amino acids), and a short intracellular domain (26 amino acids). The extracellular domain is sometimes referred to as EpEX, and the intracellular domain is sometimes referred to as EpICD.

Function

The exact function of EpCAM is currently being elucidated, but EpCAM appears to play many different roles.

Cell adhesion

EpCAM was first found to play a role in homotypic cell adhesion. This means that EpCAM on the surface of one cell binds to the EpCAM on a neighboring cell thereby holding the cells together. The adhesions mediated by EpCAM are relatively weak, as compared to some other adhesion molecules, such as classic cadherins.

EpICD is required for EpCAM to mediate intercellular adhesion; EpCAM mediates intercellular adhesion and associates with the actin cytoskeleton via EpICD.[12]

EpCAM has a negative impact on cadherin-mediated adhesions. Overexpression of EpCAM does not alter overall total cellular level of cadherins but rather decreases the association of the cadherin/ catenin complex in the cytoskeleton. As EpCAM expression increases, the total amount of α-catenin decreases, whereas cellular β-catenin levels remain constant.[13]

The homotypic adhesive activity has been questioned, as a variety of in vivo and in vitro biochemical experiments have failed to detect trans-interactions.[14] EpCAM pro-adhesive activity could be explained by alternative models,[15] based on its ability to regulate PKC signalling and myosin activity.[16]

Recently, it has been discovered that EpCAM contributes to the maintenance of tight junctions.[17]

Active proliferation in a number of epithelial tissues is associated with increased or de novo EpCAM expression. This is especially evident in tissues that normally reveal no or low levels of EpCAM expression, such as squamous epithelium. The level of EpCAM expression correlates with the proliferative activity of intestinal cells, and inversely correlates with their differentiation.

Role in cancer

EpCAM can be cleaved which lends the molecule oncogenic potential. Upon cleavage, the extracellular domain (EpEX) is released into the area surrounding the cell, and the intracellular domain (EpICD) is released into the cytoplasm of the cell. EpICD forms a complex with the proteins FHL2, β-catenin, and Lef inside the nucleus. This complex then binds to DNA and promotes the transcription of various genes. Targets of upregulation include c-myc, e-fabp, and cyclins A & E. This has the effect of promoting tumor growth. Additionally, EpEX that has been cleaved can stimulate the cleavage of additional EpCAM molecules resulting in a positive feedback loop. The amount of β-catenin in the nucleus can modulate the expression level of EpCAM.[18]

EpCAM may also play a role in epithelial mesenchymal transition (EMT) in tumors, although its exact effects are poorly understood. Its ability to suppress E-cadherin suggests that EpCAM would promote EMT and tumor metastasis, but its homotypic cell adhesion properties can counteract its ability to suppress E-cadherin.[19] Results from different studies are often conflicting. In one study, for example, silencing of EpCAM with short interfering RNA (siRNA) led to a reduction of proliferation, migration, and invasion of breast cancer cells in vitro supporting the role of EpCAM in promoting EMT. In another study, cells undergoing EMT were found to downregulate EpCAM.[20] In one study, epithelial tumors were often strongly positive for EpCAM, but mesenchymal tumors showed only occasional and weak positivity. It has been suggested that EpCAM expression is downregulated during EMT but then upregulated once the metastasis reaches its future tumor site.[21]

Clinical significance

Target for immunotherapy

It has been speculated that since EpCAM in normal epithelia is expressed mostly on the basolateral membrane, it would be much less accessible to antibodies than EpCAM in cancer tissue, where it is homogeneously distributed on the cancer cell surface. In addition to being overexpressed in many carcinomas, EpCAM is expressed in cancer stem cells, making EpCAM an attractive target for immunotherapy. However, the heterogeneous expression of EpCAM in carcinomas and the fact that EpCAM is not tumor-specific (i.e., it is found in normal epithelium) raise concerns that immunotherapy directed towards EpCAM could have severe side effects. As the role of EpCAM in cancer cell signaling is better understood, EpCAM signaling rather than EpCAM itself may be a target for therapeutic intervention.

Edrecolomab, catumaxomab, nofetumomab and other monoclonal antibodies are designed to bind to it.[22]

Histopathology

EpCAM is often overexpressed in certain carcinomas, including in breast cancer, colon cancer and basal cell carcinoma of the skin.[23] The diagnosis of such conditions can therefore be assisted by immunohistochemistry using BerEp4, which is an antibody to EpCAM.[23]

Genetic disorders

A problem in EpCAM can indirectly cause Lynch syndrome,[24] a genetic disorder that leads to increased risk of cancer. Deletion of a portion of the 3' end of the EpCAM gene causes epigenetic inactivation of the MSH2 gene by hypermethylating the promoter region of the MSH2 gene.

Mutations in EpCAM have also been associated with congenital tufting enteropathy[25] which causes intractable diarrhea in newborn children.

External links

Notes and References

  1. Litvinov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO . Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule . The Journal of Cell Biology . 125 . 2 . 437–46 . April 1994 . 8163559 . 2120036 . 10.1083/jcb.125.2.437 .
  2. Maetzel D, Denzel S, Mack B, Canis M, Went P, Benk M, Kieu C, Papior P, Baeuerle PA, Munz M, Gires O . 6 . Nuclear signalling by tumour-associated antigen EpCAM . Nature Cell Biology . 11 . 2 . 162–71 . February 2009 . 19136966 . 10.1038/ncb1824 . 8616872 .
  3. Osta WA, Chen Y, Mikhitarian K, Mitas M, Salem M, Hannun YA, Cole DJ, Gillanders WE . 6 . EpCAM is overexpressed in breast cancer and is a potential target for breast cancer gene therapy . Cancer Research . 64 . 16 . 5818–24 . August 2004 . 15313925 . 10.1158/0008-5472.CAN-04-0754 . free .
  4. Litvinov SV, van Driel W, van Rhijn CM, Bakker HA, van Krieken H, Fleuren GJ, Warnaar SO . Expression of Ep-CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation . The American Journal of Pathology . 148 . 3 . 865–75 . March 1996 . 8774141 . 1861708 .
  5. Münz M, Kieu C, Mack B, Schmitt B, Zeidler R, Gires O . The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation . Oncogene . 23 . 34 . 5748–58 . July 2004 . 15195135 . 10.1038/sj.onc.1207610 . 32348616 . free .
  6. Armstrong A, Eck SL . EpCAM: A new therapeutic target for an old cancer antigen . Cancer Biology & Therapy . 2 . 4 . 320–6 . 2003 . 14508099 . 10.4161/cbt.2.4.451 . free .
  7. Herlyn D, Herlyn M, Steplewski Z, Koprowski H . Monoclonal antibodies in cell-mediated cytotoxicity against human melanoma and colorectal carcinoma . European Journal of Immunology . 9 . 8 . 657–9 . August 1979 . 499332 . 10.1002/eji.1830090817 . 28251532 .
  8. Baeuerle PA, Gires O . EpCAM (CD326) finding its role in cancer . British Journal of Cancer . 96 . 3 . 417–23 . February 2007 . 17211480 . 2360029 . 10.1038/sj.bjc.6603494 .
  9. Balzar M, Winter MJ, de Boer CJ, Litvinov SV . The biology of the 17-1A antigen (Ep-CAM) . Journal of Molecular Medicine . 77 . 10 . 699–712 . October 1999 . 10606205 . 10.1007/s001099900038 . 13253137 .
  10. Munz M, Baeuerle PA, Gires O . The emerging role of EpCAM in cancer and stem cell signaling . Cancer Research . 69 . 14 . 5627–9 . July 2009 . 19584271 . 10.1158/0008-5472.CAN-09-0654 . free .
  11. Went PT, Lugli A, Meier S, Bundi M, Mirlacher M, Sauter G, Dirnhofer S . Frequent EpCam protein expression in human carcinomas . Human Pathology . 35 . 1 . 122–8 . January 2004 . 14745734 . 10.1016/j.humpath.2003.08.026 .
  12. Balzar M, Bakker HA, Briaire-de-Bruijn IH, Fleuren GJ, Warnaar SO, Litvinov SV . Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule . Molecular and Cellular Biology . 18 . 8 . 4833–43 . August 1998 . 9671492 . 109068 . 10.1128/MCB.18.8.4833 .
  13. Litvinov SV, Balzar M, Winter MJ, Bakker HA, Briaire-de Bruijn IH, Prins F, Fleuren GJ, Warnaar SO . 6 . Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins . The Journal of Cell Biology . 139 . 5 . 1337–48 . December 1997 . 9382878 . 2140211 . 10.1083/jcb.139.5.1337 .
  14. Gaber A, Kim SJ, Kaake RM, Benčina M, Krogan N, Šali A, Pavšič M, Lenarčič B . 6 . EpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion . Scientific Reports . 8 . 1 . 13269 . September 2018 . 30185875 . 6125409 . 10.1038/s41598-018-31482-7 . 2018NatSR...813269G .
  15. Fagotto F, Aslemarz A . EpCAM cellular functions in adhesion and migration, and potential impact on invasion: A critical review . Biochimica et Biophysica Acta (BBA) - Reviews on Cancer . 1874 . 2 . 188436 . September 2020 . 32976980 . 10.1016/j.bbcan.2020.188436 . 221939186 . free .
  16. Maghzal N, Kayali HA, Rohani N, Kajava AV, Fagotto F . EpCAM controls actomyosin contractility and cell adhesion by direct inhibition of PKC . Developmental Cell . 27 . 3 . 263–77 . November 2013 . 24183651 . 10.1016/j.devcel.2013.10.003 . free .
  17. Varadarajan S, Raya-Sandino A, Nusrat A . Clipping EpCAM to release Claudin-7 for the greater good of the epithelial barrier . The Journal of Cell Biology . 222 . 1 . e202211127 . December 2022 . 36516449 . 10.1083/jcb.202211127 . 9754701 . free .
  18. Yamashita T, Budhu A, Forgues M, Wang XW . Activation of hepatic stem cell marker EpCAM by Wnt-beta-catenin signaling in hepatocellular carcinoma . Cancer Research . 67 . 22 . 10831–9 . November 2007 . 18006828 . 10.1158/0008-5472.CAN-07-0908 . free .
  19. Patriarca C, Macchi RM, Marschner AK, Mellstedt H . Epithelial cell adhesion molecule expression (CD326) in cancer: a short review . Cancer Treatment Reviews . 38 . 1 . 68–75 . February 2012 . 21576002 . 10.1016/j.ctrv.2011.04.002 .
  20. Santisteban M, Reiman JM, Asiedu MK, Behrens MD, Nassar A, Kalli KR, Haluska P, Ingle JN, Hartmann LC, Manjili MH, Radisky DC, Ferrone S, Knutson KL . 6 . Immune-induced epithelial to mesenchymal transition in vivo generates breast cancer stem cells . Cancer Research . 69 . 7 . 2887–95 . April 2009 . 19276366 . 2664865 . 10.1158/0008-5472.CAN-08-3343 .
  21. van der Gun BT, Melchers LJ, Ruiters MH, de Leij LF, McLaughlin PM, Rots MG . EpCAM in carcinogenesis: the good, the bad or the ugly . Carcinogenesis . 31 . 11 . 1913–21 . November 2010 . 20837599 . 10.1093/carcin/bgq187 . free .
  22. Punt CJ, Nagy A, Douillard JY, Figer A, Skovsgaard T, Monson J, Barone C, Fountzilas G, Riess H, Moylan E, Jones D, Dethling J, Colman J, Coward L, MacGregor S . 6 . Edrecolomab alone or in combination with fluorouracil and folinic acid in the adjuvant treatment of stage III colon cancer: a randomised study . Lancet . 360 . 9334 . 671–7 . August 2002 . 12241873 . 10.1016/S0140-6736(02)09836-7 . 42391189 .
  23. Dasgeb B, Mohammadi TM, Mehregan DR . Use of Ber-EP4 and Epithelial Specific Antigen to Differentiate Clinical Simulators of Basal Cell Carcinoma . Biomarkers in Cancer . 5 . 7–11 . 2013 . 24179394 . 10.4137/BIC.S11856 . 3791948 . free .
  24. Tomita N, Yamano T, Matsubara N, Tamura K . [A novel genetic disorder of Lynch syndrome - EPCAM gene deletion] . Gan to Kagaku Ryoho. Cancer & Chemotherapy . 40 . 2 . 143–7 . February 2013 . 23411950 .
  25. Sivagnanam M, Mueller JL, Lee H, Chen Z, Nelson SF, Turner D, Zlotkin SH, Pencharz PB, Ngan BY, Libiger O, Schork NJ, Lavine JE, Taylor S, Newbury RO, Kolodner RD, Hoffman HM . 6 . Identification of EpCAM as the gene for congenital tufting enteropathy . Gastroenterology . 135 . 2 . 429–37 . August 2008 . 18572020 . 2574708 . 10.1053/j.gastro.2008.05.036 .