| Hebname: | עֵין הַבְּשׂוֹר |
| Imgsize: | 250px |
| Foundation: | 1982 |
| Founded By: | Evacuated settlers |
| District: | south |
| Council: | Eshkol |
| Affiliation: | Moshavim Movement |
| Pushpin Map: | Israel northwest negev#Israel |
| Pushpin Mapsize: | 250 |
| Pushpin Label Position: | bottom |
| Coordinates: | 31.2822°N 34.4503°W |
Ein HaBesor (he|עֵין הַבְּשׂוֹר||Spring of the [[Besor]]) is a moshav in southern Israel. Located in the Hevel Eshkol area of the north-western Negev desert near the border with the Gaza Strip and around a kilometre from Magen, it falls under the jurisdiction of Eshkol Regional Council. In it had a population of .
Ein Besor was an Egyptian First Dynasty staging post along the "ways of Horus" trade route in the northern Negev. The staging post was contemporary with Tell es-Sakan.[1] [2] The modern moshav was established in 1982. Some of the residents were from, an Israeli settlement in the Sinai Peninsula evacuated after signing of the Egypt–Israel peace treaty.[3]
On 7 October 2023, Ein HaBesor was attacked by Hamas fighters. The moshav's civil defense team, with only four M16 rifles, repelled the attack.[4]