Efferocytosis Explained

In cell biology, efferocytosis (from efferre, Latin for 'to carry out'[1] (to the grave), extended meaning 'to bury') is the process by which apoptotic cells are removed by phagocytic cells. It can be regarded as the 'burying of dead cells'.[2] [3]

During efferocytosis, the cell membrane of phagocytic cells engulfs the apoptotic cell, forming a large fluid-filled vesicle containing the dead cell. This ingested vesicle is called an efferosome (in analogy to the term phagosome). This process is similar to macropinocytosis.

Mechanism

For apoptosis, the effect of efferocytosis is that dead cells are removed before their membrane integrity is breached and their contents leak into the surrounding tissue. This prevents exposure of tissue to toxic enzymes, oxidants and other intracellular components such as proteases and caspases.[4]

Efferocytosis can be performed not only by 'professional' phagocytic cells such as macrophages or dendritic cells, but also by many other cell types including epithelial cells and fibroblasts. To distinguish them from living cells, apoptotic cells carry specific 'eat me' signals, such as the presence of phosphatidyl serine (resulting from phospholipid flip-flop) or calreticulin on the outer leaflet of the cell membrane.[5]

Down stream consequences

Efferocytosis triggers specific downstream intracellular signal transduction pathways, for example resulting in anti-inflammatory, anti-protease and growth-promoting effects. Conversely, impaired efferocytosis has been linked to autoimmune disease and tissue damage. Efferocytosis results in production by the ingesting cell of mediators such as hepatocyte- and vascular endothelial growth factor, which are thought to promote replacement of the dead cells.[4]

Specialized pro-resolving mediators are cell-derived metabolites of certain polyunsaturated fatty acids viz.: arachidonic acid which is metabolized to the lipoxins; eicosapentaenoic acid which is metabolized to the Resolvin E's; docosahexaenoic acid which is metabolized to the resolvin D's, maresins, and neuroprotectins; and n-3 docosapentaenoic acid which is metabolized to the n-3 docosapentaenoic acid-derived resolvins and n-3 docosapentaenoic acid-derived neuroprotectins (See Specialized pro-resolving mediators). These mediators possess a broad range of overlapping activities which act to resolve inflammation; one of the important activities which many of these mediators possess is the stimulation of efferocytosis in inflamed tissues.[6] [7] [8] Failure to form sufficient amounts of these mediators is proposed to be one cause of chronic and pathological inflammatory responses (see Specialized pro-resolving mediators#SPM and inflammation).

Clincal significance

Defective efferocytosis has been demonstrated in such diseases as cystic fibrosis and bronchiectasis, Chronic obstructive pulmonary disease, asthma and idiopathic pulmonary fibrosis, rheumatoid arthritis, systemic lupus erythematosus, glomerulonephritis and atherosclerosis.[4]

Notes and References

  1. Book: Simpson, D.P. . Cassell's Latin Dictionary . Bloomsbury Publishing . 2000 . 9780826430212 . 208.
  2. deCathelineau AM, Henson PM . The final step in programmed cell death: phagocytes carry apoptotic cells to the grave . Essays in Biochemistry . 39 . 105–117 . 2003 . 14585077 . 10.1042/bse0390105 .
  3. Kojima Y, Weissman IL, Leeper NJ . The Role of Efferocytosis in Atherosclerosis . Circulation . 135 . 5 . 476–489 . January 2017 . 28137963 . 5302553 . 10.1161/CIRCULATIONAHA.116.025684 .
  4. Vandivier RW, Henson PM, Douglas IS . Burying the dead: the impact of failed apoptotic cell removal (efferocytosis) on chronic inflammatory lung disease . Chest . 129 . 6 . 1673–1682 . June 2006 . 16778289 . 10.1378/chest.129.6.1673 .
  5. Gardai SJ, McPhillips KA, Frasch SC, Janssen WJ, Starefeldt A, Murphy-Ullrich JE, Bratton DL, Oldenborg PA, Michalak M, Henson PM . 6 . Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte . Cell . 123 . 2 . 321–334 . October 2005 . 16239148 . 10.1016/j.cell.2005.08.032 . 18123002 . free .
  6. Haworth O, Buckley CD . Pathways involved in the resolution of inflammatory joint disease . Seminars in Immunology . 27 . 3 . 194–199 . May 2015 . 25944272 . 10.1016/j.smim.2015.04.002 .
  7. Shinohara M, Serhan CN . Novel Endogenous Proresolving Molecules:Essential Fatty Acid-Derived and Gaseous Mediators in the Resolution of Inflammation . Journal of Atherosclerosis and Thrombosis . 23 . 6 . 655–664 . June 2016 . 27052783 . 7399282 . 10.5551/jat.33928 . free .
  8. Basil MC, Levy BD . Specialized pro-resolving mediators: endogenous regulators of infection and inflammation . Nature Reviews. Immunology . 16 . 1 . 51–67 . January 2016 . 26688348 . 5242505 . 10.1038/nri.2015.4 .