| Season: | 2000–01 |
| Total Goals: | 1018 |
| Average Goals: | 3.32 |
| Nextseason: | 2001–02 |
The Dutch Eerste Divisie in the 2000–01 season was contested by 18 teams. FC Den Bosch won the championship.
Relegated from the 1999–2000 Eredivisie
In the promotion/relegation competition, eight entrants (six from this league and two from the Eredivisie) entered in two groups. The group winners were promoted to the Eredivisie.