Edith Wilson Miles Explained

Edith Wilson Miles
Birth Name:Edith Margaret Wilson
Alma Mater:University of California, Berkeley
Thesis Title:The bacterial metabolism of [alpha]-methylserine and hydroxymethylserine
Thesis Url:https://www.worldcat.org/oclc/
Thesis Year:1962

Edith Wilson Miles (born Edith Margaret Wilson) is a biochemist known for her work on the structure and function of enzymes, especially her work on tryptophan synthase.

Education and career

Miles received her B.A. from the University of Texas at Austin in 1957, and then moved to the University of California, Berkeley where she earned a Ph.D. in 1962[1] working in Esmond Emerson Snell's lab with Jesse Rabinowitz and Edward Adelberg as her advisors.[2] [3] With funding from the American Cancer Society, she moved to the University of Leicester as a postdoctoral researcher with Hans Kornberg. From 1964 until 1966, she was a postdoctoral investigator at Tufts University working with Alton Meister, and then she accepted an independent position at the National Institutes of Health. In 2000 she became a Scientist Emeritus.

Research

Wilson's graduate research characterized an enzyme that required pyridoxal phosphate and tetrahydrofolate to convert -methylserine to alanine and formaldehyde.[4] [5] Her subsequent work examined the glyoxylate cycle in bacterial cells and led to further investigation of enzymes that require pyridoxal phosphate.[6] Upon her move to the National Institutes of Health, she began to focus on tryptophan synthase,[7] [8] [9] first by establishing the mechanism of the enzyme[10] which would later allow her to investigate interactions between the subunits of the enzyme. Wilson went on to use x-ray crystallography to obtain the structure of the enzyme,[11] [12] and used mutant forms of Salmonella typhimurium to identify the significant components of the enzyme. She also showed that complex of tryptophan synthase could unfold in the presence of guanine hydrochloride,[13] details about protein folding and shape that became relevant in later research about barrel-shaped proteins.[14] [15]

Selected publications

Awards and honors

While at the University of Texas at Austin, Miles (then known as Edith Margaret Wilson) was inducted into Alpha Lambda Delta,[16] [17] an honor society that recognizes achievement of first year university students and for which she later served as secretary.[18] In her senior year, 1957, she was elected to Phi Beta Kappa[19] and was a member of Mortar Board. In 1994, Miles received the Hillebrand Award, named for William Francis Hillebrand, from the Chemical Society of Washington, a section of the American Chemical Society.[20]

Personal life

Her husband, H. Todd Miles, also worked at the National Institutes of Health and became Scientist Emeritus in 2000.

Notes and References

  1. Miles. Edith Wilson. April 5, 2013. The Tryptophan Synthase α2β2 Complex: A Model for Substrate Channeling, Allosteric Communication, and Pyridoxal Phosphate Catalysis. Journal of Biological Chemistry. English. 288. 14. 10084–10091. 10.1074/jbc.X113.463331. 0021-9258. 3617248. 23426371. free.
  2. Snell. Esmond E.. 1993. FROM BACTERIAL NUTRITION TO ENZYME STRUCTURE: A Personal Odyssey. Annual Review of Biochemistry. en. 62. 1. 1–26. 10.1146/annurev.bi.62.070193.000245. 8352584. 0066-4154. free.
  3. Miles. Edith Wilson. Metzler. David E.. November 1, 2004. Esmond Emerson Snell (1914–2003). The Journal of Nutrition. 134. 11. 2907–2910. 10.1093/jn/134.11.2907. 15514250. 0022-3166. free.
  4. Wilson. Edith M.. Snell. Esmond E.. 1962. Metabolism of α-Methylserine. Journal of Biological Chemistry. 237. 10. 3171–3179. 10.1016/s0021-9258(18)50139-3. 0021-9258. free.
  5. Wilson. Edith M.. Snell. Esmond E.. 1962. Metabolism of α-Methylserine. Journal of Biological Chemistry. 237. 10. 3180–3184. 10.1016/s0021-9258(18)50140-x. 0021-9258. free.
  6. WILSON. EM. KORNBERG. HL. September 1, 1963. PROPERTIES OF CRYSTALLINE l-ASPARTATE 4-CARBOXY-LYASE FROM ACHROMOBACTER SP.. Biochemical Journal. 88. 3. 578–587. 10.1042/bj0880578. 0006-2936. 1202217. 14071532.
  7. Book: Miles, Edith Wilson. Advances in enzymology : and related areas of molecular biology. Vol.64. 1991. NewYork ; Chichester . Wiley. 978-0-471-50949-3. 64. 93–172. Structural basis for catalysis by tryptophan synthase.
  8. Book: Miles, Edith Wilson. Advances in enzymology : and related areas of molecular biology. Vol.64. 1991. NewYork ; Chichester . Wiley. 978-0-471-50949-3. 49. 127–. Tryptophan synthase: structure, function, and subunit interaction.
  9. Book: Subcellular biochemistry. Vol.24, Proteins: structure, function and engineering. 1995. New York ; London . Plenum. 978-0-306-44846-1. 207–254. Chapter 8 Tryptophan synthase structure, function, and protein engineering.
  10. Miles. Edith Wilson.. Hatanaka. M.. Crawford. I. P.. August 1, 1968. A new thiol-dependent transamination reaction catalyzed by the B protein of Escherichia coli tryptophan synthetase. Biochemistry. en. 7. 8. 2742–2753. 10.1021/bi00848a008. 4877071. 0006-2960.
  11. Ahmed. S A. Miles. E W. Davies. D R. 1985. Crystallization and preliminary X-ray crystallographic data of the tryptophan synthase alpha 2 beta 2 complex from Salmonella typhimurium.. Journal of Biological Chemistry. 260. 6. 3716–3718. 10.1016/s0021-9258(19)83682-7. 3882715 . 0021-9258. free.
  12. Hyde. C C. Ahmed. S A. Padlan. E A. Miles. E W. Davies. D R. 1988. Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium.. Journal of Biological Chemistry. 263. 33. 17857–17871. 10.1016/s0021-9258(19)77913-7. 3053720 . 0021-9258. free.
  13. Miles. Edith Wilson. Yutani. Katsuhide. Ogasahara. Kyoko. May 25, 1982. Guanidine hydrochloride-induced unfolding of the .alpha. subunit of tryptophan synthase and of the two .alpha. proteolytic fragments: evidence for stepwise unfolding of the two .alpha. domains. Biochemistry. en. 21. 11. 2586–2592. 10.1021/bi00540a002. 7046790. 0006-2960.
  14. Miles. Edith Wilson. Davies. David R.. 2000. On the Ancestry of Barrels. Science. 289. 5484. 1490. 10.1126/science.289.5484.1490. 3077778. 10991737. 82637907. 0036-8075.
  15. 1978. Protein Clipping in Lab and Cell. Science News. 113. 17. 279–280. 10.2307/3962872. 3962872. 0036-8423.
  16. Web site: Alpha Lambda Delta - Honorary scholastic fraternity for freshman. live. January 9, 2022. University of Texas at Austin. https://web.archive.org/web/20220109175329/https://repositories.lib.utexas.edu/bitstream/handle/2152/61518/University_of_Texas_Austin_TX_1956.txt?sequence=3&isAllowed=y . January 9, 2022 .
  17. Book: Cactus Yearbook, 1955. 1955. University of Texas at Austin. 283. 10.15781/T2ZG6GP6H. University Of Texas At Austin.
  18. Book: Cactus Yearbook, 1956. 1956. University of Texas at Austin. 167. 10.15781/T23776B01. University Of Texas At Austin.
  19. Book: Cactus Yearbook, 1957. 1957. University of Texas at Austin. 167. 10.15781/T23776B01. 2152/61517. University Of Texas At Austin.
  20. 2009. August/September 2009. Past Awardees of the CSW Hillebrand Award. 59. 6. January 9, 2022. The Capital Chemist.

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