EcoEI R protein C-terminal domain explained

Symbol:	EcoEI_R_C
EcoEI_R_C
Pfam:	PF08463
Interpro:	IPR013670

In molecular biology, the EcoEI R protein C-terminal domain is a protein domain found at the C-terminus of both the R subunit of type I restriction enzymes and the Res subunit of type III restriction enzymes. The type I enzymes include EcoEI, which recognises 5'-GAGN(7)ATGC-3; the R protein (HsdR) is required for both nuclease and ATPase activity.^{[1] [2]}

Notes and References

1. Murray NE, Daniel AS, Cowan GM, Sharp PM . Conservation of motifs within the unusually variable polypeptide sequences of type I restriction and modification enzymes . Mol. Microbiol. . 9 . 1 . 133–43 . July 1993 . 8412658 . 10.1111/j.1365-2958.1993.tb01675.x. 45127462 .
2. Makovets S, Doronina VA, Murray NE . Regulation of endonuclease activity by proteolysis prevents breakage of unmodified bacterial chromosomes by type I restriction enzymes . Proc. Natl. Acad. Sci. U.S.A. . 96 . 17 . 9757–62 . August 1999 . 10449767 . 22283 . 10.1073/pnas.96.17.9757. 1999PNAS...96.9757M . free .