ELOB explained

Elongin B is a protein that in humans is encoded by the ELOB gene.^[1]

Function

Elongin B is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.^[2]

Interactions

TCEB2 has been shown to interact with:

• CUL2,^[3]
• TCEB1,^{[4] [5] [6] [7]} and
• Von Hippel-Lindau tumor suppressor.^{[8] [5] [9] [10] [11]}

Further reading

• Duan DR, Pause A, Burgess WH, Aso T, Chen DY, Garrett KP, Conaway RC, Conaway JW, Linehan WM, Klausner RD . Inhibition of transcription elongation by the VHL tumor suppressor protein . Science . 269 . 5229 . 1402–6 . 1995 . 7660122 . 10.1126/science.7660122 . 1995Sci...269.1402D . 22334719 .
• Aso T, Lane WS, Conaway JW, Conaway RC . Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II . Science . 269 . 5229 . 1439–43 . 1995 . 7660129 . 10.1126/science.7660129 . 1995Sci...269.1439A . 37649908 .
• Kibel A, Iliopoulos O, DeCaprio JA, Kaelin WG . Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C . Science . 269 . 5229 . 1444–6 . 1995 . 7660130 . 10.1126/science.7660130 . 1995Sci...269.1444K . 25410108 .
• Bradsher JN, Jackson KW, Conaway RC, Conaway JW . RNA polymerase II transcription factor SIII. I. Identification, purification, and properties . J. Biol. Chem. . 268 . 34 . 25587–93 . 1994 . 10.1016/S0021-9258(19)74431-7 . 8244996 . free .
• Pause A, Lee S, Worrell RA, Chen DY, Burgess WH, Linehan WM, Klausner RD . The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins . Proc. Natl. Acad. Sci. U.S.A. . 94 . 6 . 2156–61 . 1997 . 9122164 . 20057 . 10.1073/pnas.94.6.2156 . 1997PNAS...94.2156P . free .
• Li XY, Green MR . The HIV-1 Tat cellular coactivator Tat-SF1 is a general transcription elongation factor . Genes Dev. . 12 . 19 . 2992–6 . 1998 . 9765201 . 317190 . 10.1101/gad.12.19.2992 .
• Zhang JG, Farley A, Nicholson SE, Willson TA, Zugaro LM, Simpson RJ, Moritz RL, Cary D, Richardson R, Hausmann G, Kile BJ, Kent SB, Alexander WS, Metcalf D, Hilton DJ, Nicola NA, Baca M . The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradation . Proc. Natl. Acad. Sci. U.S.A. . 96 . 5 . 2071–6 . 1999 . 10051596 . 26738 . 10.1073/pnas.96.5.2071 . 1999PNAS...96.2071Z . free .
• Stebbins CE, Kaelin WG, Pavletich NP . Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function . Science . 284 . 5413 . 455–61 . 1999 . 10205047 . 10.1126/science.284.5413.455 . 1999Sci...284..455S .
• Pause A, Peterson B, Schaffar G, Stearman R, Klausner RD . Studying interactions of four proteins in the yeast two-hybrid system: structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein . Proc. Natl. Acad. Sci. U.S.A. . 96 . 17 . 9533–8 . 1999 . 10449727 . 22243 . 10.1073/pnas.96.17.9533 . 1999PNAS...96.9533P . free .
• Iwai K, Yamanaka K, Kamura T, Minato N, Conaway RC, Conaway JW, Klausner RD, Pause A . Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex . Proc. Natl. Acad. Sci. U.S.A. . 96 . 22 . 12436–41 . 1999 . 10535940 . 22941 . 10.1073/pnas.96.22.12436 . 1999PNAS...9612436I . free .
• Ohh M, Takagi Y, Aso T, Stebbins CE, Pavletich NP, Zbar B, Conaway RC, Conaway JW, Kaelin WG . Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein . J. Clin. Invest. . 104 . 11 . 1583–91 . 2000 . 10587522 . 481054 . 10.1172/JCI8161 .
• Aso T, Yamazaki K, Amimoto K, Kuroiwa A, Higashi H, Matsuda Y, Kitajima S, Hatakeyama M . Identification and characterization of Elongin A2, a new member of the Elongin family of transcription elongation factors, specifically expressed in the testis . J. Biol. Chem. . 275 . 9 . 6546–52 . 2000 . 10692460 . 10.1074/jbc.275.9.6546 . free .
• Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW . Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex . Proc. Natl. Acad. Sci. U.S.A. . 97 . 19 . 10430–5 . 2000 . 10973499 . 27041 . 10.1073/pnas.190332597 . 2000PNAS...9710430K . free .
• Aso T, Yamazaki K, Aigaki T, Kitajima S . Drosophila von Hippel-Lindau tumor suppressor complex possesses E3 ubiquitin ligase activity . Biochem. Biophys. Res. Commun. . 276 . 1 . 355–61 . 2000 . 11006129 . 10.1006/bbrc.2000.3451 .
• Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW . Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase . J. Biol. Chem. . 276 . 32 . 29748–53 . 2001 . 11384984 . 10.1074/jbc.M103093200 . free .
• Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G . Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein . J. Biol. Chem. . 277 . 7 . 4656–62 . 2002 . 11739384 . 10.1074/jbc.M108269200 . free .
• Yamazaki K, Guo L, Sugahara K, Zhang C, Enzan H, Nakabeppu Y, Kitajima S, Aso T . Identification and biochemical characterization of a novel transcription elongation factor, Elongin A3 . J. Biol. Chem. . 277 . 29 . 26444–51 . 2002 . 11994304 . 10.1074/jbc.M202859200 . free .
• Min JH, Yang H, Ivan M, Gertler F, Kaelin WG, Pavletich NP . Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling . Science . 296 . 5574 . 1886–9 . 2002 . 12004076 . 10.1126/science.1073440 . 2002Sci...296.1886M . 19641938 . free .
• Hon WC, Wilson MI, Harlos K, Claridge TD, Schofield CJ, Pugh CW, Maxwell PH, Ratcliffe PJ, Stuart DI, Jones EY . Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL . Nature . 417 . 6892 . 975–8 . 2002 . 12050673 . 10.1038/nature00767 . 2002Natur.417..975H . 4388644 .

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Elongin-B

Notes and References

1. Garrett KP, Aso T, Bradsher JN, Foundling SI, Lane WS, Conaway RC, Conaway JW . Positive regulation of general transcription factor SIII by a tailed ubiquitin homolog . Proc Natl Acad Sci U S A . 92 . 16 . 7172–6 . September 1995 . 7638163 . 41301 . 10.1073/pnas.92.16.7172 . 1995PNAS...92.7172G . free .
2. Web site: ELOB elongin B [Homo sapiens (human) ] ].
3. Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW . Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase . J. Biol. Chem. . 276 . 32 . 29748–53 . August 2001 . 11384984 . 10.1074/jbc.M103093200 . free .
4. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . October 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
5. Ohh M, Takagi Y, Aso T, Stebbins CE, Pavletich NP, Zbar B, Conaway RC, Conaway JW, Kaelin WG . Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein . J. Clin. Invest. . 104 . 11 . 1583–91 . Dec 1999 . 10587522 . 481054 . 10.1172/JCI8161 .
6. Krumm A, Groudine M . Tumor suppression and transcription elongation: the dire consequences of changing partners . Science . 269 . 5229 . 1400–1 . September 1995 . 7660121 . 10.1126/science.7660121. 1995Sci...269.1400K . 39758696 .
7. Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G . Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein . J. Biol. Chem. . 277 . 7 . 4656–62 . February 2002 . 11739384 . 10.1074/jbc.M108269200 . free .
8. Menon S, Tsuge T, Dohmae N, Takio K, Wei N . Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome . BMC Biochem. . 9 . 1 . 18173839 . 2265268 . 10.1186/1471-2091-9-1 . 2008 . free .
9. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 89 . 17353931 . 1847948 . 10.1038/msb4100134 . 2007 .
10. Min JH, Yang H, Ivan M, Gertler F, Kaelin WG, Pavletich NP . Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling . Science . 296 . 5574 . 1886–9 . June 2002 . 12004076 . 10.1126/science.1073440 . 2002Sci...296.1886M . 19641938 . free .
11. Hacker KE, Lee CM, Rathmell WK . VHL type 2B mutations retain VBC complex form and function . PLOS ONE . 3 . 11 . e3801 . 19030229 . 2583047 . 10.1371/journal.pone.0003801 . Zhang . Baohong . 2008 . 2008PLoSO...3.3801H . free .