EIF2C2 explained

Protein argonaute-2 is a protein that in humans is encoded by the EIF2C2 gene.^{[1] [2] [3]}

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with Dicer1 and play a role in short-interfering-RNA-mediated gene silencing.^[4]

Interactions

EIF2C2 has been shown to interact with

• DDX20,^{[5] [6]}
• DICER1,^{[6] [7]}
• FMRP, FXR1P, FXR2P,^[8] and
• TNRC6B.^[9]

Further reading

• Carmell MA, Xuan Z, Zhang MQ, Hannon GJ . The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. . Genes Dev. . 16 . 21 . 2733–42 . 2002 . 12414724 . 10.1101/gad.1026102 . free .
• Mourelatos Z, Dostie J, Paushkin S . miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. . Genes Dev. . 16 . 6 . 720–8 . 2002 . 11914277 . 10.1101/gad.974702 . 155365 . etal.
• Martinez J, Patkaniowska A, Urlaub H . Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. . Cell . 110 . 5 . 563–74 . 2003 . 12230974 . 10.1016/S0092-8674(02)00908-X . etal. 11858/00-001M-0000-0012-F2FD-2 . 10616773 . free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Doi N, Zenno S, Ueda R . Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. . Curr. Biol. . 13 . 1 . 41–6 . 2003 . 12526743 . 10.1016/S0960-9822(02)01394-5 . 14650075 . etal. free . 2003CBio...13...41D .
• Tahbaz N, Kolb FA, Zhang H . Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer. . EMBO Rep. . 5 . 2 . 189–94 . 2004 . 14749716 . 10.1038/sj.embor.7400070 . 1298981 . etal.
• Nelson PT, Hatzigeorgiou AG, Mourelatos Z . miRNP:mRNA association in polyribosomes in a human neuronal cell line. . RNA . 10 . 3 . 387–94 . 2004 . 14970384 . 10.1261/rna.5181104 . 1370934 .
• Liu J, Carmell MA, Rivas FV . Argonaute2 is the catalytic engine of mammalian RNAi. . Science . 305 . 5689 . 1437–41 . 2004 . 15284456 . 10.1126/science.1102513 . 2004Sci...305.1437L . 2778088 . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Rivas FV, Tolia NH, Song JJ . Purified Argonaute2 and an siRNA form recombinant human RISC. . Nat. Struct. Mol. Biol. . 12 . 4 . 340–9 . 2005 . 15800637 . 10.1038/nsmb918 . 2021813 . etal.
• Sen GL, Blau HM . Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. . Nat. Cell Biol. . 7 . 6 . 633–6 . 2005 . 15908945 . 10.1038/ncb1265 . 6085169 .
• Liu J, Valencia-Sanchez MA, Hannon GJ, Parker R . MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. . Nat. Cell Biol. . 7 . 7 . 719–23 . 2005 . 15937477 . 10.1038/ncb1274 . 1855297 .
• Rual JF, Venkatesan K, Hao T . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 . etal.
• Kraynack BA, Baker BF . Small interfering RNAs containing full 2'-O-methylribonucleotide-modified sense strands display Argonaute2/eIF2C2-dependent activity. . RNA . 12 . 1 . 163–76 . 2006 . 16301602 . 10.1261/rna.2150806 . 1370895 .
• Maniataki E, Mourelatos Z . A human, ATP-independent, RISC assembly machine fueled by pre-miRNA. . Genes Dev. . 19 . 24 . 2979–90 . 2006 . 16357216 . 10.1101/gad.1384005 . 1315402 .
• Shi H, Tschudi C, Ullu E . Functional replacement of Trypanosoma brucei Argonaute by the human slicer Argonaute2. . RNA . 12 . 6 . 943–7 . 2006 . 16611939 . 10.1261/rna.20806 . 1464859 .
• Wichroski MJ, Robb GB, Rana TM . Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies. . PLOS Pathog. . 2 . 5 . e41 . 2006 . 16699599 . 10.1371/journal.ppat.0020041 . 1458959 . free .

Notes and References

1. Koesters R, Adams V, Betts D, Moos R, Schmid M, Siermann A, Hassam S, Weitz S, Lichter P, Heitz PU, von Knebel Doeberitz M, Briner J . Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression . Genomics . 61 . 2 . 210–8 . Dec 1999 . 10534406 . 10.1006/geno.1999.5951 . 24461432 .
2. Sasaki T, Shiohama A, Minoshima S, Shimizu N . Identification of eight members of the Argonaute family in the human genome small star, filled . Genomics . 82 . 3 . 323–30 . Aug 2003 . 12906857 . 10.1016/S0888-7543(03)00129-0 .
3. Web site: Entrez Gene: EIF2C2 eukaryotic translation initiation factor 2C, 2.
4. Web site: Entrez Gene: EIF2C2 eukaryotic translation initiation factor 2C, 2.
5. Nelson PT, Hatzigeorgiou AG, Mourelatos Z . miRNP:mRNA association in polyribosomes in a human neuronal cell line . RNA . 10 . 3 . 387–94 . March 2004 . 14970384 . 1370934 . 10.1261/rna.5181104.
6. Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G . miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs . Genes Dev. . 16 . 6 . 720–8 . March 2002 . 11914277 . 155365 . 10.1101/gad.974702 .
7. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K . Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors . Curr. Biol. . 13 . 1 . 41–6 . January 2003 . 12526743 . 10.1016/S0960-9822(02)01394-5. 14650075 . free . 2003CBio...13...41D .
8. Jin P, Zarnescu DC, Ceman S, Nakamoto M, Mowrey J, Jongens TA, Nelson DL, Moses K, Warren ST . Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway . Nat. Neurosci. . 7 . 2 . 113–7 . February 2004 . 14703574 . 10.1038/nn1174 . 1146182 .
9. Till S, Lejeune E, Thermann R, Bortfeld M, Hothorn M, Enderle D, Heinrich C, Hentze MW, Ladurner AG . A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain . Nat. Struct. Mol. Biol. . 14 . 10 . 897–903 . October 2007 . 17891150 . 10.1038/nsmb1302 . 8136380 .