EEF1B2 explained

Elongation factor 1-beta is a protein that in humans is encoded by the EEF1B2 gene.^{[1] [2]}

Function

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Interactions

EEF1B2 has been shown to interact with EEF1G^{[3] [4]} and HARS.^[5]

Further reading

• Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J . Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes . Electrophoresis . 13 . 12 . 960–9 . Dec 1992 . 1286667 . 10.1002/elps.11501301199 . 41855774 .
• Hochstrasser DF, Frutiger S, Paquet N, Bairoch A, Ravier F, Pasquali C, Sanchez JC, Tissot JD, Bjellqvist B, Vargas R . Human liver protein map: a reference database established by microsequencing and gel comparison . Electrophoresis . 13 . 12 . 992–1001 . Dec 1992 . 1286669 . 10.1002/elps.11501301201 . 23518983 .
• von der Kammer H, Klaudiny J, Zimmer M, Scheit KH . Human elongation factor 1 beta: cDNA and derived amino acid sequence . Biochemical and Biophysical Research Communications . 177 . 1 . 312–7 . May 1991 . 1710449 . 10.1016/0006-291X(91)91984-K .
• Sanders J, Maassen JA, Amons R, Möller W . Nucleotide sequence of human elongation factor-1 beta cDNA . Nucleic Acids Research . 19 . 16 . 4551 . Aug 1991 . 1886777 . 328648 . 10.1093/nar/19.16.4551 .
• Bec G, Kerjan P, Zha XD, Waller JP . Valyl-tRNA synthetase from rabbit liver. I. Purification as a heterotypic complex in association with elongation factor 1 . The Journal of Biological Chemistry . 264 . 35 . 21131–7 . Dec 1989 . 10.1016/S0021-9258(19)30056-0 . 2556394 . free .
• Wolfson AD, Orlovsky AF, Gladilin KL . Mammalian valyl-tRNA synthetase forms a complex with the first elongation factor . FEBS Letters . 238 . 2 . 262–4 . Oct 1988 . 3169261 . 10.1016/0014-5793(88)80492-7 . 45934458 . free . 1988FEBSL.238..262M .
• Chen CJ, Traugh JA . Expression of recombinant elongation factor 1 beta from rabbit in Escherichia coli. Phosphorylation by casein kinase II . Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression . 1264 . 3 . 303–11 . Dec 1995 . 8547318 . 10.1016/0167-4781(95)00166-2 .
• Béhar G, Coleclough C, Houlgatte R, Auffray C, Lefkovits I . Human lymphocyte cDNA ordered library analyzed by 2D gel electrophoresis. 3. Analysis of individual clones . Applied and Theoretical Electrophoresis . 5 . 2 . 99–105 . 1996 . 8573604 .
• Sanders J, Brandsma M, Janssen GM, Dijk J, Möller W . Immunofluorescence studies of human fibroblasts demonstrate the presence of the complex of elongation factor-1 beta gamma delta in the endoplasmic reticulum . Journal of Cell Science . 109 (Pt 5) . 5 . 1113–7 . May 1996 . 10.1242/jcs.109.5.1113 . 8743958 . 1854/LU-01GJAXGWEXX2G4TPB1AKB720BQ .
• Sheu GT, Traugh JA . Recombinant subunits of mammalian elongation factor 1 expressed in Escherichia coli. Subunit interactions, elongation activity, and phosphorylation by protein kinase CKII . The Journal of Biological Chemistry . 272 . 52 . 33290–7 . Dec 1997 . 9407120 . 10.1074/jbc.272.52.33290 . free .
• Sheu GT, Traugh JA . A structural model for elongation factor 1 (EF-1) and phosphorylation by protein kinase CKII . Molecular and Cellular Biochemistry . 191 . 1–2 . 181–6 . Jan 1999 . 10094407 . 10.1023/A:1006802125856 . 775166 .
• Pérez JM, Siegal G, Kriek J, Hård K, Dijk J, Canters GW, Möller W . The solution structure of the guanine nucleotide exchange domain of human elongation factor 1beta reveals a striking resemblance to that of EF-Ts from Escherichia coli . Structure . 7 . 2 . 217–26 . Feb 1999 . 10368288 . 10.1016/S0969-2126(99)80027-6 . free .
• Chambers DM, Rouleau GA, Abbott CM . Comparative genomic analysis of genes encoding translation elongation factor 1B(alpha) in human and mouse shows EEF1B1 to be a recent retrotransposition event . Genomics . 77 . 3 . 145–8 . Oct 2001 . 11597139 . 10.1006/geno.2001.6626 .
• Sang Lee J, Gyu Park S, Park H, Seol W, Lee S, Kim S . Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex . Biochemical and Biophysical Research Communications . 291 . 1 . 158–64 . Feb 2002 . 11829477 . 10.1006/bbrc.2002.6398 .
• Cans C, Passer BJ, Shalak V, Nancy-Portebois V, Crible V, Amzallag N, Allanic D, Tufino R, Argentini M, Moras D, Fiucci G, Goud B, Mirande M, Amson R, Telerman A . Translationally controlled tumor protein acts as a guanine nucleotide dissociation inhibitor on the translation elongation factor eEF1A . Proceedings of the National Academy of Sciences of the United States of America . 100 . 24 . 13892–7 . Nov 2003 . 14623968 . 283517 . 10.1073/pnas.2335950100 . 2003PNAS..10013892C . free .
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proceedings of the National Academy of Sciences of the United States of America . 101 . 33 . 12130–5 . Aug 2004 . 15302935 . 514446 . 10.1073/pnas.0404720101 . 2004PNAS..10112130B . free .
• Ito T, Marintchev A, Wagner G . Solution structure of human initiation factor eIF2alpha reveals homology to the elongation factor eEF1B . Structure . 12 . 9 . 1693–704 . Sep 2004 . 15341733 . 10.1016/j.str.2004.07.010 . free .

Notes and References

1. Pizzuti A, Gennarelli M, Novelli G, Colosimo A, Lo Cicero S, Caskey CT, Dallapiccola B . Human elongation factor EF-1 beta: cloning and characterization of the EF1 beta 5a gene and assignment of EF-1 beta isoforms to chromosomes 2,5,15 and X . Biochemical and Biophysical Research Communications . 197 . 1 . 154–62 . Nov 1993 . 8250921 . 10.1006/bbrc.1993.2454 .
2. Web site: Entrez Gene: EEF1B2 eukaryotic translation elongation factor 1 beta 2.
3. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
4. Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE . A human protein-protein interaction network: a resource for annotating the proteome . Cell . 122 . 6 . 957–68 . Sep 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . 11858/00-001M-0000-0010-8592-0 . 8235923 . free .
5. Sang Lee J, Gyu Park S, Park H, Seol W, Lee S, Kim S . Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex . Biochemical and Biophysical Research Communications . 291 . 1 . 158–64 . Feb 2002 . 11829477 . 10.1006/bbrc.2002.6398 .