EEF1A2 explained

Elongation factor 1-alpha 2 is a protein that in humans is encoded by the EEF1A2 gene.^{[1] [2] [3]}

Function

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas.

Clinical significance

This gene may be critical in the development of ovarian cancer.

Regulation

EEF1A2 is a direct target of miRNA-663 and miRNA-744.^[4]

Further reading

• Lee JM . The role of protein elongation factor eEF1A2 in ovarian cancer . Reprod. Biol. Endocrinol. . 1 . 69 . 2004 . 14588074 . 239897 . 10.1186/1477-7827-1-69 . free .
• Beh KJ . Immunoglobulin class specificity of non-agglutinating antibody produced in cattle following Brucella abortus 45/20 vaccination . Aust. Vet. J. . 51 . 10 . 481–3 . 1976 . 812466 . 10.1111/j.1751-0813.1975.tb02385.x .
• Lee S, Ann DK, Wang E . Cloning of human and mouse brain cDNAs coding for S1, the second member of the mammalian elongation factor-1 alpha gene family: analysis of a possible evolutionary pathway . Biochem. Biophys. Res. Commun. . 203 . 3 . 1371–7 . 1994 . 7945283 . 10.1006/bbrc.1994.2336 .
• Richard Gaynor . Wu-Baer F, Lane WS, Gaynor RB . Identification of a group of cellular cofactors that stimulate the binding of RNA polymerase II and TRP-185 to human immunodeficiency virus 1 TAR RNA . J. Biol. Chem. . 271 . 8 . 4201–8 . 1996 . 8626763 . 10.1074/jbc.271.8.4201 . free .
• Bischoff C, Kahns S, Lund A, Jørgensen HF, Praestegaard M, Clark BF, Leffers H . The human elongation factor 1 A-2 gene (EEF1A2): complete sequence and characterization of gene structure and promoter activity . Genomics . 68 . 1 . 63–70 . 2001 . 10950927 . 10.1006/geno.2000.6271.
• McClatchy DB, Knudsen CR, Clark BF, Kahn RA, Hall RA, Levey AI . Novel interaction between the M4 muscarinic acetylcholine receptor and elongation factor 1A2 . J. Biol. Chem. . 277 . 32 . 29268–74 . 2002 . 12048193 . 10.1074/jbc.M203081200. free .
• Anand N, Murthy S, Amann G, Wernick M, Porter LA, Cukier IH, Collins C, Gray JW, Diebold J, Demetrick DJ, Lee JM . Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer . Nat. Genet. . 31 . 3 . 301–5 . 2002 . 12053177 . 10.1038/ng904. 37997742 .
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G . A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway . Nat. Cell Biol. . 6 . 2 . 97–105 . 2004 . 14743216 . 10.1038/ncb1086. 11683986 .
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells . Nat. Biotechnol. . 23 . 1 . 94–101 . 2005 . 15592455 . 10.1038/nbt1046. 7200157 .
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207. 2005Natur.433...77A . 4344740 .
• Byun HM, Suh D, Jeong Y, Wee HS, Kim JM, Kim WK, Ko JJ, Kim JS, Lee YB, Oh YK . Plasmid vectors harboring cellular promoters can induce prolonged gene expression in hematopoietic and mesenchymal progenitor cells . Biochem. Biophys. Res. Commun. . 332 . 2 . 518–23 . 2005 . 15893736 . 10.1016/j.bbrc.2005.04.155.
• Ahmed M, Forsberg J, Bergsten P . Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry . J. Proteome Res. . 4 . 3 . 931–40 . 2005 . 15952740 . 10.1021/pr050024a .
• Tomlinson VA, Newbery HJ, Wray NR, Jackson J, Larionov A, Miller WR, Dixon JM, Abbott CM . Translation elongation factor eEF1A2 is a potential oncoprotein that is overexpressed in two-thirds of breast tumours . BMC Cancer . 5 . 113 . 2006 . 16156888 . 1236916 . 10.1186/1471-2407-5-113 . free .
• Li R, Wang H, Bekele BN, Yin Z, Caraway NP, Katz RL, Stass SA, Jiang F . Identification of putative oncogenes in lung adenocarcinoma by a comprehensive functional genomic approach . Oncogene . 25 . 18 . 2628–35 . 2006 . 16369491 . 10.1038/sj.onc.1209289. 3101952 .

Notes and References

1. Knudsen SM, Frydenberg J, Clark BF, Leffers H . Tissue-dependent variation in the expression of elongation factor-1 alpha isoforms: isolation and characterisation of a cDNA encoding a novel variant of human elongation-factor 1 alpha . Eur J Biochem . 215 . 3 . 549–54 . Sep 1993 . 8354261 . 10.1111/j.1432-1033.1993.tb18064.x . free .
2. Lund A, Knudsen SM, Vissing H, Clark B, Tommerup N . Assignment of human elongation factor 1alpha genes: EEF1A maps to chromosome 6q14 and EEF1A2 to 20q13.3 . Genomics . 36 . 2 . 359–61 . Jan 1997 . 8812466 . 10.1006/geno.1996.0475 .
3. Web site: Entrez Gene: EEF1A2 eukaryotic translation elongation factor 1 alpha 2.
4. Vislovukh A, Kratassiouk G, Porto E, Gralievska N, Beldiman C, Pinna G, El'skaya A, Harel-Bellan A, Negrutskii B, Groisman I . Proto-oncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR-663 and miR-744. . British Journal of Cancer . 108 . 11 . 2304–11 . 11 June 2013 . 23695020 . 10.1038/bjc.2013.243 . 3681015.