Cucumisin Explained

Cucumisin
Ec Number:	3.4.21.25
Cas Number:	82062-89-3

Cucumisin (euphorbain, solanain, hurain, tabernamontanain) is an enzyme.^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyzes hydrolysis of a wide range of proteins. It has been identified as an allergen in humans.^[8]

This enzyme is isolated from the sarcocarp of the musk melon (Cucumis melo).

Notes and References

1. Plant proteases. I. Activation-inhibition reactions . Greenberg DM, Winnick T . J. Biol. Chem. . 1940 . 135 . 761–773 .
2. Hurain, a new plant protease from Hura crepitans . Jaffé WG . J. Biol. Chem. . 1943 . 149 . 1–7 .
3. A new vegetable proteolytic enzyme of the papain class . Jaffé WG . Rev. Brasil Biol. . 1943 . 3 . 149–157 .
4. Kaneda M, Tominaga N . Isolation and characterization of a proteinase from the sarcocarp of melon fruit . Journal of Biochemistry . 78 . 6 . 1287–96 . December 1975 . 5423 .
5. Kaneda M, Ohmine H, Yonezawa H, Tominaga N . Amino acid sequence around the reactive serine of cucumisin from melon fruit . Journal of Biochemistry . 95 . 3 . 825–9 . March 1984 . 6427203 .
6. Two proteases from the latex of Elaeophorbia drupifera . Lynn KR, Clevette-Radford NA . Phytochemistry . 1985 . 24 . 2843–2845 . 10.1016/0031-9422(85)80011-x.
7. Specificity of cucumisin in hydrolysis of peptide thiobenzyl esters . Kaneda N, Minematsu Y, Powers JC, Tominaga N . Agric. Biol. Chem. . 1986 . 50 . 1075–1076 . 10.1271/bbb1961.50.1075. free .
8. Molecular Cloning and Expression of Cucumisin (Cuc m 1), a Subtilisin-like Protease of Cucumis melo in Escherichia coli . Sankian M, Talebi F, Moghadam M, Vahedi F, Jabbari Azad F, Varasteh A . Allergology International . 2011 . 60 . 1 . 61–67 . 10.2332/allergolint.10-OA-0195. free .