(KDO)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase explained

(KDO)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase
Ec Number:	2.4.99.13

(KDO)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (KDO transferase, waaA (gene), kdtA (gene), Kdo transferase, 3-deoxy-D-manno-oct-2-ulosonic acid transferase, 3-deoxy-manno-octulosonic acid transferase) is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:(KDO)-lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase.^{[1] [2]} This enzyme catalyses the following chemical reaction

alpha-Kdo-(2->6)-lipid IVA + CMP-alpha-Kdo

\rightleftharpoons alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + CMP

The bifunctional enzyme from Escherichia coli transfers two 3-deoxy-D-manno-oct-2-ulosonate residues to lipid IVA.

See also

• EC 2.4.99.12, lipid IVA 3-deoxy-D-manno-octulosonic acid transferase

Notes and References

1. Belunis CJ, Raetz CR . Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli . The Journal of Biological Chemistry . 267 . 14 . 9988–97 . May 1992 . 1577828 .
2. Löbau S, Mamat U, Brabetz W, Brade H . Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-alpha-D-manno-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183 . Molecular Microbiology . 18 . 3 . 391–9 . November 1995 . 8748024 . 10.1111/j.1365-2958.1995.mmi_18030391.x .