E2F5 explained

Transcription factor E2F5 is a protein that in humans is encoded by the E2F5 gene.^{[1] [2]}

Function

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.

Interactions

E2F5 has been shown to interact with TFDP1.^[3]

See also

• E2F

Further reading

• Hijmans EM, Voorhoeve PM, Beijersbergen RL, van 't Veer LJ, Bernards R . E2F-5, a new E2F family member that interacts with p130 in vivo . Molecular and Cellular Biology . 15 . 6 . 3082–9 . Jun 1995 . 7760804 . 230539 . 10.1128/mcb.15.6.3082.
• Ginsberg D, Vairo G, Chittenden T, Xiao ZX, Xu G, Wydner KL, DeCaprio JA, Lawrence JB, Livingston DM . E2F-4, a new member of the E2F transcription factor family, interacts with p107 . Genes & Development . 8 . 22 . 2665–79 . Nov 1994 . 7958924 . 10.1101/gad.8.22.2665 . free .
• Beijersbergen RL, Kerkhoven RM, Zhu L, Carlée L, Voorhoeve PM, Bernards R . E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo . Genes & Development . 8 . 22 . 2680–90 . Nov 1994 . 7958925 . 10.1101/gad.8.22.2680 . free .
• Itoh A, Levinson SF, Morita T, Kourembanas S, Brody JS, Mitsialis SA . Structural characterization and specificity of expression of E2F-5: a new member of the E2F family of transcription factors . Cellular & Molecular Biology Research . 41 . 3 . 147–54 . 1996 . 8589754 .
• Lindeman GJ, Gaubatz S, Livingston DM, Ginsberg D . The subcellular localization of E2F-4 is cell-cycle dependent . Proceedings of the National Academy of Sciences of the United States of America . 94 . 10 . 5095–100 . May 1997 . 9144196 . 24637 . 10.1073/pnas.94.10.5095 . 1997PNAS...94.5095L . free .
• Vaishnav YN, Vaishnav MY, Pant V . The molecular and functional characterization of E2F-5 transcription factor . Biochemical and Biophysical Research Communications . 242 . 3 . 586–92 . Jan 1998 . 9464260 . 10.1006/bbrc.1997.8010 .
• Morris L, Allen KE, La Thangue NB . Regulation of E2F transcription by cyclin E-Cdk2 kinase mediated through p300/CBP co-activators . Nature Cell Biology . 2 . 4 . 232–9 . Apr 2000 . 10783242 . 10.1038/35008660 . 36134762 .
• Gaubatz S, Lindeman GJ, Ishida S, Jakoi L, Nevins JR, Livingston DM, Rempel RE . E2F4 and E2F5 play an essential role in pocket protein-mediated G1 control . Molecular Cell . 6 . 3 . 729–35 . Sep 2000 . 11030352 . 10.1016/S1097-2765(00)00071-X . free .
• D'Souza SJ, Pajak A, Balazsi K, Dagnino L . Ca2+ and BMP-6 signaling regulate E2F during epidermal keratinocyte differentiation . The Journal of Biological Chemistry . 276 . 26 . 23531–8 . Jun 2001 . 11319226 . 10.1074/jbc.M100780200 . free .
• Chestukhin A, Litovchick L, Rudich K, DeCaprio JA . Nucleocytoplasmic shuttling of p130/RBL2: novel regulatory mechanism . Molecular and Cellular Biology . 22 . 2 . 453–68 . Jan 2002 . 11756542 . 139733 . 10.1128/MCB.22.2.453-468.2002 .
• Apostolova MD, Ivanova IA, Dagnino C, D'Souza SJ, Dagnino L . Active nuclear import and export pathways regulate E2F-5 subcellular localization . The Journal of Biological Chemistry . 277 . 37 . 34471–9 . Sep 2002 . 12089160 . 10.1074/jbc.M205827200 . free .
• Chen CR, Kang Y, Siegel PM, Massagué J . E2F4/5 and p107 as Smad cofactors linking the TGFbeta receptor to c-myc repression . Cell . 110 . 1 . 19–32 . Jul 2002 . 12150994 . 10.1016/S0092-8674(02)00801-2 . 8945574 . free .
• Macaluso M, Cinti C, Russo G, Russo A, Giordano A . pRb2/p130-E2F4/5-HDAC1-SUV39H1-p300 and pRb2/p130-E2F4/5-HDAC1-SUV39H1-DNMT1 multimolecular complexes mediate the transcription of estrogen receptor-alpha in breast cancer . Oncogene . 22 . 23 . 3511–7 . Jun 2003 . 12789259 . 10.1038/sj.onc.1206578 . free .
• Ohtani N, Brennan P, Gaubatz S, Sanij E, Hertzog P, Wolvetang E, Ghysdael J, Rowe M, Hara E . Epstein-Barr virus LMP1 blocks p16INK4a-RB pathway by promoting nuclear export of E2F4/5 . The Journal of Cell Biology . 162 . 2 . 173–83 . Jul 2003 . 12860972 . 2172795 . 10.1083/jcb.200302085 .
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW . Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation . Nature Biotechnology . 22 . 6 . 707–16 . Jun 2004 . 15146197 . 10.1038/nbt971 . 27764390 .
• Jiang Y, Saavedra HI, Holloway MP, Leone G, Altura RA . Aberrant regulation of survivin by the RB/E2F family of proteins . The Journal of Biological Chemistry . 279 . 39 . 40511–20 . Sep 2004 . 15271987 . 10.1074/jbc.M404496200 . free .
• Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S . Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions . Genome Research . 14 . 9 . 1711–8 . Sep 2004 . 15342556 . 515316 . 10.1101/gr.2435604 .
• Joshi B, Ordonez-Ercan D, Dasgupta P, Chellappan S . Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors . Oncogene . 24 . 13 . 2204–17 . Mar 2005 . 15735762 . 10.1038/sj.onc.1208206 . free .
• Swetloff A, Ferretti P . Changes in E2F5 intracellular localization in mouse and human choroid plexus epithelium with development . The International Journal of Developmental Biology . 49 . 7 . 859–65 . 2006 . 16172982 . 10.1387/ijdb.051996as . free .

Notes and References

1. Sardet C, Vidal M, Cobrinik D, Geng Y, Onufryk C, Chen A, Weinberg RA . E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle . Proceedings of the National Academy of Sciences of the United States of America . 92 . 6 . 2403–7 . Mar 1995 . 7892279 . 42492 . 10.1073/pnas.92.6.2403 . 1995PNAS...92.2403S . free .
2. Web site: Entrez Gene: E2F5 E2F transcription factor 5, p130-binding.
3. Hijmans EM, Voorhoeve PM, Beijersbergen RL, van 't Veer LJ, Bernards R . E2F-5, a new E2F family member that interacts with p130 in vivo . Molecular and Cellular Biology . 15 . 6 . 3082–9 . Jun 1995 . 7760804 . 230539 . 10.1128/mcb.15.6.3082.