Double-stranded uracil-DNA glycosylase explained

Double-stranded uracil-DNA glycosylase
Ec Number:	3.2.2.28

Double-stranded uracil-DNA glycosylase (Mug, double-strand uracil-DNA glycosylase, Dug, dsUDG, double-stranded DNA specific UDG, dsDNA specific UDG, UdgB, G:T/U mismatch-specific DNA glycosylase, UDG) is an enzyme with systematic name uracil-double-stranded DNA deoxyribohydrolase (uracil-releasing).^{[1] [2] [3]} This enzyme catalyses a specific chemical reaction: it hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free uracil.

This enzyme is not active on DNA containing a T/G mispair or single-stranded DNA.

Notes and References

1. Barrett TE, Schärer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH . Crystal structure of a thwarted mismatch glycosylase DNA repair complex . The EMBO Journal . 18 . 23 . 6599–609 . December 1999 . 10581234 . 1171723 . 10.1093/emboj/18.23.6599 . Gregory L. Verdine .
2. Sung JS, Mosbaugh DW . Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV . Biochemistry . 39 . 33 . 10224–35 . August 2000 . 10956012 . 10.1021/bi0007066 .
3. Mokkapati SK, Fernández de Henestrosa AR, Bhagwat AS . Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells . Molecular Microbiology . 41 . 5 . 1101–11 . September 2001 . 11555290 . 10.1046/j.1365-2958.2001.02559.x .