Dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase explained
| Dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase |
| Ec Number: | 2.4.1.258 |
Dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase (Man5GlcNAc2-PP-Dol mannosyltransferase, ALG3, dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase, Not56-like protein, Alg3 alpha-1,3-mannosyl transferase, Dol-P-Man:Man5GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase) is an enzyme with systematic name dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase.[1] [2] This enzyme catalyses the following chemical reaction
dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate.
References
- Rearick JI, Fujimoto K, Kornfeld S . 1981 . Identification of the mannosyl donors involved in the synthesis of lipid-linked oligosaccharides . J. Biol. Chem. . 256 . 3762 - 9 . 6163773 . 8 .
Notes and References
- Sharma CB, Knauer R, Lehle L . Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase . Biological Chemistry . 382 . 2 . 321–8 . February 2001 . 11308030 . 10.1515/bc.2001.039 .
- Cipollo JF, Trimble RB . The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing . The Journal of Biological Chemistry . 275 . 6 . 4267–77 . February 2000 . 10660594 . 10.1074/jbc.275.6.4267 . free .
