| DNA replication gene dnaC | |
| Organism: | Escherichia coli (str. K-12 substr. MG1655) |
| Taxid: | 511145 |
| Symbol: | dnaC |
| Entrezgene: | 948864 |
| Refseqprotein: | NP_418781.1 |
| Uniprot: | P0AEF0 |
| Ecnumber: | 2.7.7.7 |
| Chromosome: | genome |
| Entrezchromosome: | NC_000913.2 |
| Genloc Start: | 4598149 |
| Genloc End: | 4599108 |
dnaC is a loading factor that complexes with the C-terminus of helicase dnaB and inhibits it from unwinding the dsDNA at a replication fork.[1] A dnaB and dnaC associate near the dnaA bound origin for each of the ssDNA.[1] One dnaB-dnaC complex is oriented in the opposite direction to the other dnaB-dnaC complex due to the antiparallel nature of DNA. Because they are oriented in opposite directions, one dnaB-dnaC complex will complex with dnaA from the N-terminus of dnaB whereas the other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be allowed to begin unwinding dsDNA to make room for DNA polymerase III to begin synthesizing the daughter strands.[1]
This interaction of dnaC with dnaB requires the hydrolysis of ATP.[2]