| Jurisdiction: | Diocese |
| Kwito-Bié | |
| Latin: | Dioecesis Kvitobiensis |
| Local: | Portuguese: Diocese do Kwito-Bié |
| Country: |  Angola |
| Bishop: | Vicente Sanombo |
| Bishop Title: | Bishop-elect |
| Metropolitan: | Huambo |
| Rite: | Roman Rite |
| Particular Church: | Latin Church |
| Area Km2: | 71,000 |
| Catholics Percent: | 47 |
| Population: | 1,910,000 |
| Population As Of: | 2015 |
| Catholics: | 900,000 |
| Area Footnotes: | [1] |
The Roman Catholic Diocese of Kwito-Bié (Latin: Dioecesis Kvitobiensis) is a diocese located in Bié Province in the ecclesiastical province of Huambo in Angola. The cathedral is located in the city of Cuito.
The diocese was established on 4 September 1940 as the Diocese of Silva Porto on territory separated from the Diocese of São Paulo de Loanda. Initially, the diocese was a suffragan of the Roman Catholic Archdiocese of Luanda. The diocese lost territory on 25 November 1957 to establish the Roman Catholic Diocese of Malanje and on 1 July 1963 to establish the Diocese of Luso.
On 3 February 1977, the diocese changed suffragans to the Archdiocese of Huambo. On 16 May 1979, it was renamed as the Diocese of Kwito-Bié.
The Cathedral of the diocese is Sé Catedral de São Lourenço in Cuito, Bié Province.