| Jurisdiction: | Diocese |
| Santa Clara | |
| Latin: | Dioecesis Centumfocencis |
| Country: | Cuba |
| Metropolitan: | Santa Clara, Cuba |
| Province: | Province of Camagüey |
| Area Km2: | 12,754 |
| Population: | 1,176,219 |
| Population As Of: | 2004 |
| Catholics: | 518,935 |
| Catholics Percent: | 44.1 |
| Parishes: | 2234 |
| Denomination: | Roman Catholic |
| Rite: | Latin Rite |
| Established: | 1 April 1995 (years ago) |
| Cathedral: | Cathedral of St. Clare of Assisi |
| Bishop Title: | Bishop |
| Map: | Diócesis de Santa Clara.svg |
The Diocese of Santa Clara is a diocese of the Latin Church of the Roman Catholic Church in Cuba. The diocese is a suffragan of the Archdiocese of Camagüey. Originally erected as part of the Diocese of Cienfuegos in 1903, the parent diocese was renamed to the Diocese of Cienfuegos-Santa Clara in 1971. Finally in 1995 the parent diocese was split to form the Diocese of Cienfuegos and the Diocese of Santa Clara.