| Jurisdiction: | Diocese |
| Novo Mesto | |
| Latin: | Dioecesis Novae Urbis |
| Local: | Škofija Novo mesto |
| Country: | Slovenia |
| Established: | 7 April 2006 |
| Rite: | Latin Rite |
| Cathedral: | Saint Nicholas' Cathedral, Novo Mesto |
| Area Km2: | 2,160 |
| Population: | 159,595 |
| Population As Of: | 2013 |
| Catholics: | 137,424 |
| Catholics Percent: | 86.1 |
| Metro Archbishop: | Stane Zore |
| Website: | Website of the Diocese |
The Diocese of Novo Mesto (Latin: Dioecesis Novae Urbis; Slovenian: Škofija Novo mesto) is a Latin Church diocese of the Catholic Church in the city of Novo Mesto in the ecclesiastical province of Ljubljana in Slovenia.