| Jurisdiction: | Diocese |
| Holguín | |
| Latin: | Dioecesis Holguinensis |
| Country: |  Cuba |
| Metropolitan: | Holguín |
| Province: | Province of Santiago de Cuba |
| Area Km2: | 14,089 |
| Population: | 1,605,000 |
| Population As Of: | 2006 |
| Catholics: | 435,000 |
| Catholics Percent: | 27.1 |
| Parishes: | 28 |
| Denomination: | Roman Catholic |
| Rite: | Latin Rite |
| Established: | 8 January 1979 (years ago) |
| Cathedral: | Cathedral of St. Isidore |
| Bishop Title: | Bishop |
| Emeritus Bishops: | Héctor Luis Lucas Peña Gómez |
The Roman Catholic Diocese of Holguín (erected 8 January 1979) is a suffragan diocese of the Archdiocese of Santiago de Cuba.