| Jurisdiction: | Diocese |
| Choluteca | |
| Latin: | Dioecesis Cholutecensis |
| Border: | Catholic |
| Country: |  Honduras |
| Metropolitan: | José Vicente Nácher Tatay, CM |
| Province: | Province of Tegucigalpa |
| Coordinates: | 13.3064°N -87.1949°W |
| Area Km2: | 6,282 |
| Population: | 726,250 |
| Population As Of: | 2021 |
| Catholics: | 544,370 |
| Parishes: | 16 |
| Denomination: | Catholic Church |
| Sui Iuris Church: | Latin Church |
| Rite: | Roman Rite |
| Established: | 8 September 1964 (years ago) |
| Cathedral: | Spanish; Castilian: Catedral de la Inmaculada Concepción (Cathedral of the Immaculate Conception), Choluteca |
| Priests: | 32 (30 diocesan; 2 religious) |
| Bishop Title: | Bishop |
| Metro Archbishop: | Jose Vicente Nacher Tatay |
| Map: | Diocese of Choluteca HN.svg |
The Diocese of Choluteca (erected 8 September 1964, as the Territorial Prelature of Choluteca) is a Latin Catholic diocese in Honduras. It is a suffragan diocese of the Archdiocese of Tegucigalpa. Choluteca was elevated to a diocese on 29 August 1979.