Delta8-fatty-acid desaturase explained

Delta8-fatty-acid desaturase
Ec Number:	1.14.19.4

Delta8-fatty-acid desaturase (Delta8-sphingolipid desaturase, EFD1, BoDES8, SLD, Delta8 fatty acid desaturase, Delta8-desaturase) is an enzyme with systematic name phytosphinganine,hydrogen donor:oxygen Delta8-oxidoreductase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

phytosphinganine + reduced acceptor + O₂

\rightleftharpoons Delta8-phytosphingenine + acceptor + 2 H₂O

This enzyme, which has been found mainly in plants, introduces a double bond at Delta8 of C18 and C20 fatty acids.

Notes and References

1. Wallis JG, Browse J . The Delta8-desaturase of Euglena gracilis: an alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids . Archives of Biochemistry and Biophysics . 365 . 2 . 307–16 . May 1999 . 10328826 . 10.1006/abbi.1999.1167 .
2. Sperling P, Libisch B, Zähringer U, Napier JA, Heinz E . Functional identification of a delta8-sphingolipid desaturase from Borago officinalis . Archives of Biochemistry and Biophysics . 388 . 2 . 293–8 . April 2001 . 11368168 . 10.1006/abbi.2001.2308 .
3. Takakuwa N, Kinoshita M, Oda Y, Ohnishi M . Isolation and characterization of the genes encoding delta(8)-sphingolipid desaturase from Saccharomyces kluyveri and Kluyveromyces lactis . Current Microbiology . 45 . 6 . 459–61 . December 2002 . 12402089 . 10.1007/s00284-002-3860-0 . 25735503 .
4. Beckmann C, Rattke J, Oldham NJ, Sperling P, Heinz E, Boland W . Characterization of a Delta8-sphingolipid desaturase from higher plants: a stereochemical and mechanistic study on the origin of E,Z isomers . Angewandte Chemie . 41 . 13 . 2298–300 . July 2002 . 12203571 . 10.1002/1521-3773(20020703)41:13<2298::AID-ANIE2298>3.0.CO;2-G .