Deinococcus Explained

Deinococcus (from the Greek, Modern (1453-);: δεινός, deinos, "dreadful, strange" and κόκκος, kókkos, "granule") is in the monotypic family Deinococcaceae, and one genus[1] of three in the order Deinococcales[2] [3] of the bacterial phylum Deinococcota highly resistant to environmental hazards. These bacteria have thick cell walls that give them Gram-positive stains, but they also include a second membrane and are therefore closer in structure to Gram-negative bacteria. Deinococcus survive when their DNA is exposed to high doses of gamma and UV radiation. Whereas other bacteria change their structure in the presence of radiation, such as by forming endospores, Deinococcus tolerate it without changing their cellular form and do not retreat into a hardened structure. They are also characterized by the presence of the carotenoid pigment deinoxanthin that give them their pink color. They are usually isolated according to these two criteria. In August 2020, scientists reported that bacteria from Earth, particularly Deinococcus bacteria, were found to survive for three years in outer space, based on studies conducted on the International Space Station. These findings support the notion of panspermia, the hypothesis that life exists throughout the Universe, distributed in various ways, including space dust, meteoroids, asteroids, comets, planetoids or contaminated spacecraft.[4] [5]

Molecular signatures

Members of Deinococcus can be distinguished from all other bacteria through molecular signatures known as conserved signature indels (CSIs) and proteins (CSPs). An earlier study on Deinococcus identified nine CSIs and 58 CSPs which were exclusively shared by members of this genus.[6] Some of the identified CSPs such as the DNA damage repair protein PprA and the single-stranded DNA-binding protein DdrB are thought to have functional roles in the DNA repair mechanism and radioresistance phenotype of Deinococcus.[6]

In a more recent work focused on DNA repair proteins an additional 22 CSIs were identified as specific to this genus, including a 30 amino acid insert in the UvrA1 protein that is suggested to play in a role in the resistance ability of Deinococcus species against radiation and oxidation damage.[7]

The uvrA1 gene in Deinococcus was found to form a novel genetic linkage with the genes of the proteins dCSP-1 (a transmembrane protein found only in Deinococcus species), DsbA and DsbB. The latter two proteins play a central role in the formation of disulfide bonds in proteins via oxidation-reduction of cysteine rich motifs (CXXC).[8] The above cluster of genes forms a novel operon unique to Deinococcus species and the encoded proteins are predicted to function together to combat against DNA damage caused by reactive oxidative species from radiation.[7]

The 30 aa CSI present in UvrA1 and another 5-7 aa CSI present in DsbA are located on surface loops of the proteins. The surface exposed loops/patches formed by these CSIs are thought to mediate protein-protein interactions with the transmembrane protein dCSP-1, thus facilitating a sequence of electron transfers that ultimately ameliorates oxidative damage.[7]

Comparative genomics

Although all species of the genus Deinococcus are related by definition, they exhibit substantial differences across their genomes. Most species appear to have about 3,000 genes, but only a fraction of them are shared in other species. For example, a 3-species comparison among D. radiodurans, D. deserti, and D. geothermalis shows that about two thirds of each genome is shared by all three species, but close to a third is specific and only found in one of the species (see figure). Once more genomes are included in such comparisons, the core genome will almost certainly be much smaller.

Taxonomy

See also: Bacterial taxonomy. The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) [9] and National Center for Biotechnology Information (NCBI).[10], there were 47 species of Deinococcus described.

Phylogeny

Species incertae sedis:

See also

Notes and References

  1. Brooks BW, Murray RGE (1981) Nomenclature for" Micrococcus radiodurans" and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. International Journal of Systematic and Evolutionary Microbiology 31: 353.
  2. Ekman JV, Raulio M, Busse HJ, Fewer DP, Salkinoja-Salonen M (2010) Deinobacterium chartae gen. nov., sp. nov., an extremely radiation resistant biofilm-forming bacterium isolated from a Finnish paper mill. International Journal of Systematic and Evolutionary Microbiology.
  3. Albuquerque L, Sims C, Nobre MF, Pino NM, Battista JR, et al. (2005) Truepera radiovictrix gen. nov., sp. nov., a new radiation-resistant species and the proposal of Trueperaceae fam. nov. FEMS Microbiology Letters 247: 161-169.
  4. News: Strickland . Ashley . Bacteria from Earth can survive in space and could endure the trip to Mars, according to new study . 26 August 2020 . . 26 August 2020 .
  5. Kawaguchi, Yuko . et al. . DNA Damage and Survival Time Course of Deinococcal Cell Pellets During 3 Years of Exposure to Outer Space . 26 August 2020 . . 11 . 2050 . 10.3389/fmicb.2020.02050 . 32983036 . 7479814 . free .
  6. Ho. Jonathan. Adeolu. Mobolaji. Khadka. Bijendra. Gupta. Radhey S.. October 2016. Identification of distinctive molecular traits that are characteristic of the phylum "Deinococcus–Thermus" and distinguish its main constituent groups. Systematic and Applied Microbiology. 39. 7. 453–463. 10.1016/j.syapm.2016.07.003. 27506333 . 0723-2020.
  7. Hassan. F. M. Nazmul. Gupta. Radhey S.. 2018-03-08. Novel Sequence Features of DNA Repair Genes/Proteins from Deinococcus Species Implicated in Protection from Oxidatively Generated Damage. Genes. 9. 3. 149. 10.3390/genes9030149. 29518000 . 2073-4425. 5867870. free .
  8. Inaba. Kenji. Ito. Koreaki. April 2008. Structure and mechanisms of the DsbB–DsbA disulfide bond generation machine. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1783. 4. 520–529. 10.1016/j.bbamcr.2007.11.006. 18082634 . 0167-4889. free.
  9. Web site: J.P. Euzéby . Deinococcus . 2022-07-20 . List of Prokaryotic names with Standing in Nomenclature (LPSN).
  10. Web site: Sayers. etal. DeinococcusThermus . 2022-07-20 . National Center for Biotechnology Information (NCBI) taxonomy database.
  11. Web site: The LTP . 20 November 2023.
  12. Web site: LTP_all tree in newick format. 20 November 2023.
  13. Web site: LTP_08_2023 Release Notes. 20 November 2023.
  14. Web site: GTDB release 08-RS214 . Genome Taxonomy Database. 10 May 2023.
  15. Web site: bac120_r214.sp_label . Genome Taxonomy Database. 10 May 2023.
  16. Web site: Taxon History . Genome Taxonomy Database. 10 May 2023.