David Klenerman Explained

Honorific Prefix:Sir
David Klenerman
Birth Date:[1]
Nationality:British
Fields:Biophysical chemistry
Workplaces:University of Cambridge
Stanford University
Alma Mater:University of Cambridge (MA, PhD)
Thesis Title:Infrared chemiluminescence using a SISAM spectrometer
Thesis Url:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355881
Thesis Year:1985
Doctoral Advisor:Ian William Murison Smith
Academic Advisors:Richard Zare
Known For:Illumina dye sequencing
Scanning ion-conductance microscopy
Super-resolution microscopy
Solexa
Children:2 (Laura & Anna)

Sir David Klenerman [2] [3] (born 1959) is a British biophysical chemist and a professor of biophysical chemistry at the Department of Chemistry at the University of Cambridge[4] and a Fellow of Christ's College, Cambridge.[5]

He is best known for his contribution in the field of next-generation sequencing of DNA (that subsequently resulted in Solexa, a high-speed DNA sequencing company that he co-founded),[1] [6] [7] [8] [9] nanopipette-based scanning ion-conductance microscopy,[10] [11] and super-resolution microscopy.[12]

Early life and education

Klenerman is the son of two South African-born Jews.[13] He was educated at the University of Cambridge where he was an undergraduate student of Christ's College, Cambridge and received his BA degree in 1982. He earned his PhD degree in chemistry in 1986 as a postgraduate student of Churchill College, Cambridge and was supervised by Ian William Murison Smith.[14]

Career and research

After his doctorate, Klenerman went to Stanford University as a Fulbright scholar to work on high-overtone chemistry, with Richard Zare. After his postdoctoral research at Stanford, he returned to United Kingdom to work in BP Research for seven years. Then, in 1994, he joined the University of Cambridge, as a faculty member of the Department of Chemistry and a fellow of Christ's College.[15]

Klenerman, along with Shankar Balasubramanian, invented a method of next-generation DNA sequencing which is commonly known today as the Solexa sequencing or Illumina dye sequencing. The method is based on the detection of fluorophore labelled nucleotides as they get incorporated in the DNA strands.[16] This sequencing by synthesis method gained popularity,[17] [18] and is currently regarded as the most widely used platform to replace conventional Sanger sequencing technique, despite its comparatively low multiplexing capability of samples, as it offers several key advantages: it is automated, quick, highly accurate, capable of sequencing multiple strands simultaneously via massive parallel sequencing, and economically cheaper in case of whole genome sequencing.[19] [20] [21]

He is also known for exploring nanopipette-based (instead of conventional micropipette-based) scanning ion-conductance microscopy methods.[22] His research group was successful in achieving very high resolution topographic images of live-cells, in hopping mode imaging, in precise delivery of small molecules to cell, and in studying real time detailed cell-functioning.[23] [24]

Most recently, his group is focusing on 3D super-resolution microscopy to develop new insights on protein misfolding and neurodegenerative diseases.

Commercial activities

Klenerman and Shankar Balasubramanian commercialised their invention on the single-molecule-fluorescence based high-speed DNA sequencing and jointly founded Solexa in 1998. Later, in 2007, this company was acquired by Illumina for $600 million.[25] [26] [27] [28]

In 2004, Klenerman co-founded another spin-out company, Ionscope, to supply assembled scanning ion-conductance microscopes to the research community that looks for high-resolution 3D images of live cells. As per the Biotechnology and Biological Sciences Research Council, as of February 2014, Ionscope sold 35 SICM units worldwide.[29]

Awards and honours

The major awards and honours that Klenerman received in recognition of his research work:

Notes and References

  1. Web site: David KLENERMAN - Personal Appointments (free information from Companies House). beta.companieshouse.gov.uk.
  2. Web site: David Klenerman: Royal Society. Fellows Directory . Royal Society. 2016-01-26.
  3. Web site: Fellow: Academy of Medical Sciences. Academy of Medical Sciences, United Kingdom. 26 January 2016. 26 August 2017. https://web.archive.org/web/20170826031142/https://acmedsci.ac.uk/fellows/fellows-directory/ordinary-fellows/professor-david-klenerman. dead.
  4. Web site: Professor David Klenerman FMedSci FRS. Department of Chemistry, University of Cambridge. University of Cambridge. 26 January 2016.
  5. Web site: Professor David Klenerman. Christ's College, Cambridge. 26 January 2016.
  6. Web site: History of Illumina Sequencing. Illumina. 26 January 2016.
  7. Book: Davies. Kevin. The $1,000 Genome. 7 September 2010. Free Press. 978-1416569596. 102–115. registration. 26 January 2016.
  8. Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 6 November 2008. 456. 7218. 10.1038/nature07517. 53–59. 18987734. 2581791 . Bentley . DR . Balasubramanian . S . Swerdlow . HP . et al. 2008Natur.456...53B.
  9. News: The Solexa Story. 26 January 2016. Bio-IT World. September–October 2010. 28 September 2010. 20 February 2016. https://web.archive.org/web/20160220195204/http://www.bio-itworld.com/2010/issues/sept-oct/solexa.html. dead.
  10. News: Nanodoodling shows pipette power. 26 January 2016. BBC News. 5 September 2006.
  11. News: Drawing With DNA: Nanopipette allows voltage-controlled delivery of biomolecules to a surface. 83. 26 January 2016. Chemical & Engineering News. 44. American Chemical Society. 31 October 2005.
  12. The 2015 super-resolution microscopy roadmap. Journal of Physics D. 14 October 2015. 48. 44. 10.1088/0022-3727/48/44/443001. 443001. 2015JPhD...48R3001H. 1711.04999. Hell. Stefan W.. Sahl. Steffen J.. Bates. Mark. Zhuang. Xiaowei. Heintzmann. Rainer. Booth. Martin J.. Bewersdorf. Joerg. Shtengel. Gleb. Hess. Harald. Tinnefeld. Philip. Honigmann. Alf. Jakobs. Stefan. Testa. Ilaria. Cognet. Laurent. Lounis. Brahim. Ewers. Helge. Davis. Simon J.. Eggeling. Christian. Klenerman. David. Willig. Katrin I.. Vicidomini. Giuseppe. Castello. Marco. Diaspro. Alberto. Cordes. Thorben. Steffen J. Sahl. Tinnefeld. Philip. Klenerman. David. Katrin I. Willig. 4804015.
  13. News: Frazer . Jenni . Seven Holocaust survivors on New Year's Honours List . 14 February 2024 . . 2018-12-28.
  14. PhD. University of Cambridge. Infrared chemiluminescence using a SISAM spectrometer. David. Klenerman. 1985. . lib.cam.ac.uk. 499899771.
  15. Web site: David Klenerman elected FRS. Christ's College, Cambridge. 27 January 2016. 25 August 2017. https://web.archive.org/web/20170825150633/https://www.christs.cam.ac.uk/default/news/david-klenerman-elected-frs. dead.
  16. Balasubramanian. Shankar. Sequencing nucleic acids: from chemistry to medicine. Chemical Communications. 4 May 2011. 47. 26. 7281–7286. 10.1039/c1cc11078k. 21544287. 3428630.
  17. Quail. Michael A. A large genome center's improvements to the Illumina sequencing system. Nature Methods. 25 November 2008. 5. 12. 1005–1010. 10.1038/nmeth.1270. 19034268. 2610436.
  18. Cronn. Richard. Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology. Nucleic Acids Research. 27 August 2008. 36. 19. 10.1093/nar/gkn502. e122. 18753151. 2577356.
  19. Metzker. Michael L.. Sequencing technologies – the next generation. Nature Reviews Genetics. 8 December 2009. 11. 1. 31–46. 10.1038/nrg2626. 19997069. 10.1.1.719.3885. 205484500.
  20. Pettersson. Erik. Lundeberg. Joakim. Ahmadian. Afshin. Generations of sequencing technologies. Genomics. 2009. 93. 2. 105–111. 10.1016/j.ygeno.2008.10.003. 18992322.
  21. Quail. Michael A. A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics. 24 July 2012. 13. 341. 341. 10.1186/1471-2164-13-341. 22827831. 3431227 . free .
  22. Shevchuk. Andrew I. Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe. Nanomedicine. 2011. 6. 3. 10.2217/nnm.10.154. 21542692. 565–575.
  23. Scanning Ion Conductance Microscopy. Annual Review of Analytical Chemistry. 2012. 5. 207. 207–228. 10.1146/annurev-anchem-062011-143203. 22524219. 2012ARAC....5..207C. Chen. Chiao-Chen. Zhou. Yi. Baker. Lane A..
  24. Recent advances in high resolution scanning electrochemical microscopy of living cells – A review. Analytica Chimica Acta. 2 May 2013. 775. 10.1016/j.aca.2012.12.042. 23601970. 1–13. Bergner. Stefan. Vatsyayan. Preety. Matysik. Frank-Michael. 2013AcAC..775....1B .
  25. News: Cutting the UK science budget would be a false economy. 27 January 2016. Financial Times. The Nikkei. 18 June 2013.
  26. News: $600m for DNA firm. 2016-01-27. Varsity.
  27. News: Illumina Buys Solexa. 27 January 2016. Forbes. 13 November 2006.
  28. News: Illumina to Buy Solexa For $600 Million in Stock. 27 January 2016. The Wall Street Journal. 13 November 2006.
  29. Web site: New microscope technology enables imaging of live cells. BBSRC. 30 January 2016.
  30. Web site: British Biophysical Society Lecture Tour. University College Dublin. 27 January 2016.
  31. https://royalsociety.org/grants-schemes-awards/awards/royal-medal/ Royal Medal 2018
  32. Web site: 2019 New Year Honours List. gov.uk. 31 December 2018.
  33. Web site: Breakthrough Prize – Winners Of The 2022 Breakthrough Prizes In Life Sciences, Fundamental Physics And Mathematics Announced. 13 October 2021. breakthroughprize.org. en.
  34. https://www.gairdner.org/winners Canada Gairdner International Award 2024

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