The 2000–01 Danish 1st Division season was the 56th season of the Danish 1st Division league championship and the 15th consecutive as a second tier competition governed by the Danish Football Association.
The division-champion and runner-up promoted to the 2001–02 Danish Superliga. The teams in the 14th, 15th and 16th relegated to the 2001–02 Danish 2nd Division.
| Position | Player | Club | Goals | |
|---|---|---|---|---|
| 1 | Jeppe Tengbjerg | Farum BK | 25 | |
| 2 | Tommy Olsen | Køge BK | 20 | |
| 3 | Kasper Dalgas | Vejle BK | 19 | |
| 4 | Mads Junker | BK Fremad Amager | 16 | |
| - | Martin Jeppesen | BK Frem | 16 | |
| 6 | Nicolas Drost | B 1913 | 15 | |
| - | Henrik Nielsen | Esbjerg fB | 15 | |
| 8 | Kenneth Fuhr Pedersen | Ølstykke FC | 14 | |
| - | Michael Hansen | Esbjerg fB | 14 | |
| 10 | Kim Christensen | 13 | ||
| - | Johnny Tveen | Skive IK | 13 |