DNA oxidative demethylase explained

DNA oxidative demethylase
Ec Number:	1.14.11.33

DNA oxidative demethylase (alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, alkB (gene)) is an enzyme with systematic name methyl DNA-base, 2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming).^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

DNA-base-CH₃ + 2-oxoglutarate + O₂

\rightleftharpoons DNA-base + formaldehyde + succinate + CO₂

DNA oxidative demethylase contains iron; activity is somewhat stimulated by ascorbate.

Notes and References

1. Falnes PØ, Johansen RF, Seeberg E . AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli . Nature . 419 . 6903 . 178–82 . September 2002 . 12226668 . 10.1038/nature01048 . 2002Natur.419..178F . 2372162 .
2. Yi C, Yang CG, He C . A non-heme iron-mediated chemical demethylation in DNA and RNA . Accounts of Chemical Research . 42 . 4 . 519–29 . April 2009 . 19852088 . 2920458 . 10.1021/ar800178j .
3. Yi C, Jia G, Hou G, Dai Q, Zhang W, Zheng G, Jian X, Yang CG, Cui Q, He C . Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase . Nature . 468 . 7321 . 330–3 . November 2010 . 21068844 . 3058853 . 10.1038/nature09497 . 2010Natur.468..330Y .