DNAJB1 explained

DnaJ homolog subfamily B member 1 is a protein that in humans is encoded by the DNAJB1 gene.^{[1] [2] [3]}

A fusion protein of DNAJB1 and PRKACA drives fibrolamellar hepatocellular carcinoma, a type of rare liver cancer.^[4]

Interactions

DNAJB1 has been shown to interact with:

• HSPA4,^[5] and
• STUB1^[6]

Further reading

• Hattori H, Liu YC, Tohnai I, Ueda M, Kaneda T, Kobayashi T, Tanabe K, Ohtsuka K . Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells . Cell Structure and Function . 17 . 1 . 77–86 . February 1992 . 1586970 . 10.1247/csf.17.77 . 21909960 . free .
• Raabe T, Manley JL . A human homologue of the Escherichia coli DnaJ heat-shock protein . Nucleic Acids Research . 19 . 23 . 6645 . December 1991 . 1754405 . 329243 . 10.1093/nar/19.23.6645 .
• Freeman BC, Morimoto RI . The human cytosolic molecular chaperones hsp90, hsp70 (hsc70) and hdj-1 have distinct roles in recognition of a non-native protein and protein refolding . The EMBO Journal . 15 . 12 . 2969–2979 . June 1996 . 8670798 . 450238 . 10.1002/j.1460-2075.1996.tb00660.x .
• Qian YQ, Patel D, Hartl FU, McColl DJ . Nuclear magnetic resonance solution structure of the human Hsp40 (HDJ-1) J-domain . Journal of Molecular Biology . 260 . 2 . 224–235 . July 1996 . 8764402 . 10.1006/jmbi.1996.0394 .
• Shi Y, Mosser DD, Morimoto RI . Molecular chaperones as HSF1-specific transcriptional repressors . Genes & Development . 12 . 5 . 654–666 . March 1998 . 9499401 . 316571 . 10.1101/gad.12.5.654 .
• Hata M, Ohtsuka K . Characterization of HSE sequences in human Hsp40 gene: structural and promoter analysis . Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression . 1397 . 1 . 43–55 . April 1998 . 9545528 . 10.1016/S0167-4781(97)00208-X .
• Zou J, Guo Y, Guettouche T, Smith DF, Voellmy R . Repression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1 . Cell . 94 . 4 . 471–480 . August 1998 . 9727490 . 10.1016/S0092-8674(00)81588-3 . 9234420 . free .
• Melville MW, Tan SL, Wambach M, Song J, Morimoto RI, Katze MG . The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity . The Journal of Biological Chemistry . 274 . 6 . 3797–3803 . February 1999 . 9920933 . 10.1074/jbc.274.6.3797 . free .
• Ballinger CA, Connell P, Wu Y, Hu Z, Thompson LJ, Yin LY, Patterson C . Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions . Molecular and Cellular Biology . 19 . 6 . 4535–4545 . June 1999 . 10330192 . 104411 . 10.1128/mcb.19.6.4535 .
• Michels AA, Kanon B, Bensaude O, Kampinga HH . Heat shock protein (Hsp) 40 mutants inhibit Hsp70 in mammalian cells . The Journal of Biological Chemistry . 274 . 51 . 36757–36763 . December 1999 . 10593983 . 10.1074/jbc.274.51.36757 . free .
• Terada K, Mori M . Human DnaJ homologs dj2 and dj3, and bag-1 are positive cochaperones of hsc70 . The Journal of Biological Chemistry . 275 . 32 . 24728–24734 . August 2000 . 10816573 . 10.1074/jbc.M002021200 . free .
• Ohtsuka K, Hata M . Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature . Cell Stress & Chaperones . 5 . 2 . 98–112 . April 2000 . 10.1379/1466-1268(2000)005<0098:mhdhco>2.0.co;2 . 2024-03-28 . 11147971 . 312896 .
• Kuncewicz T, Balakrishnan P, Snuggs MB, Kone BC . Specific association of nitric oxide synthase-2 with Rac isoforms in activated murine macrophages . American Journal of Physiology. Renal Physiology . 281 . 2 . F326–F336 . August 2001 . 11457725 . 10.1152/ajprenal.2001.281.2.F326 . 15719851 .
• Pang Q, Keeble W, Christianson TA, Faulkner GR, Bagby GC . FANCC interacts with Hsp70 to protect hematopoietic cells from IFN-gamma/TNF-alpha-mediated cytotoxicity . The EMBO Journal . 20 . 16 . 4478–4489 . August 2001 . 11500375 . 125562 . 10.1093/emboj/20.16.4478 .
• Hernández MP, Chadli A, Toft DO . HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor . The Journal of Biological Chemistry . 277 . 14 . 11873–11881 . April 2002 . 11809754 . 10.1074/jbc.M111445200 . free .
• Anwar A, Siegel D, Kepa JK, Ross D . Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1 . The Journal of Biological Chemistry . 277 . 16 . 14060–14067 . April 2002 . 11821413 . 10.1074/jbc.M111576200 . free .
• Reuter TY, Medhurst AL, Waisfisz Q, Zhi Y, Herterich S, Hoehn H, Gross HJ, Joenje H, Hoatlin ME, Mathew CG, Huber PA . Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport . Experimental Cell Research . 289 . 2 . 211–221 . October 2003 . 14499622 . 10.1016/S0014-4827(03)00261-1 .
• Honeyman JN, Simon EP, Robine N, Chiaroni-Clarke R, Darcy DG, Lim II, Gleason CE, Murphy JM, Rosenberg BR, Teegan L, Takacs CN, Botero S, Belote R, Germer S, Emde AK, Vacic V, Bhanot U, LaQuaglia MP, Simon SM . Detection of a recurrent DNAJB1-PRKACA chimeric transcript in fibrolamellar hepatocellular carcinoma . Science . 343 . 6174 . 1010–1014 . February 2014 . 24578576 . 4286414 . 10.1126/science.1249484 . 2014Sci...343.1010H .

Notes and References

1. Hata M, Okumura K, Seto M, Ohtsuka K . Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2 . Genomics . 38 . 3 . 446–449 . December 1996 . 8975727 . 10.1006/geno.1996.0653 .
2. Ohtsuka K . Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ . Biochemical and Biophysical Research Communications . 197 . 1 . 235–240 . November 1993 . 8250930 . 10.1006/bbrc.1993.2466 .
3. Web site: Entrez Gene: DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1.
4. Neumayer C, Ng D, Jiang CS, Qureshi A, Lalazar G, Vaughan R, Simon SM . Oncogenic Addiction of Fibrolamellar Hepatocellular Carcinoma to the Fusion Kinase DNAJB1-PRKACA . Clinical Cancer Research . 29 . 1 . 271–278 . January 2023 . 36302174 . 9811160 . 10.1158/1078-0432.CCR-22-1851 .
5. Oh WK, Song J . Cooperative interaction of Hsp40 and TPR1 with Hsp70 reverses Hsp70-HspBp1 complex formation . Molecules and Cells . 16 . 1 . 84–91 . August 2003 . 10.1016/S1016-8478(23)13770-8 . 14503850 . free .
6. Ballinger CA, Connell P, Wu Y, Hu Z, Thompson LJ, Yin LY, Patterson C . Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions . Molecular and Cellular Biology . 19 . 6 . 4535–4545 . June 1999 . 10330192 . 104411 . 10.1128/mcb.19.6.4535 .