DHX38 explained

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 is an enzyme that in humans is encoded by the DHX38 gene.^{[1] [2] [3]}

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process.^[3]

Further reading

• Schwer B, Guthrie C . PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. . Nature . 349 . 6309 . 494–9 . 1991 . 1825134 . 10.1038/349494a0 . 1991Natur.349..494S . 4281489 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery. . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Hillier LD, Lennon G, Becker M . Generation and analysis of 280,000 human expressed sequence tags. . Genome Res. . 6 . 9 . 807–28 . 1997 . 8889549 . 10.1101/gr.6.9.807 . etal. free .
• Gee S, Krauss SW, Miller E . Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43. . Proc. Natl. Acad. Sci. U.S.A. . 94 . 22 . 11803–7 . 1997 . 9342318 . 10.1073/pnas.94.22.11803 . 23596 . 1997PNAS...9411803G . etal. free .
• Wang Y, Guthrie C . PRP16, a DEAH-box RNA helicase, is recruited to the spliceosome primarily via its nonconserved N-terminal domain. . RNA . 4 . 10 . 1216–29 . 1998 . 9769096 . 10.1017/S1355838298980992 . 1369694 .
• Orphanides G, Wu WH, Lane WS . The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins. . Nature . 400 . 6741 . 284–8 . 1999 . 10421373 . 10.1038/22350 . 1999Natur.400..284O . 4300397 . etal.
• Loftus BJ, Kim UJ, Sneddon VP . Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q. . Genomics . 60 . 3 . 295–308 . 1999 . 10493829 . 10.1006/geno.1999.5927 . etal.
• Jurica MS, Licklider LJ, Gygi SR . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. . RNA . 8 . 4 . 426–39 . 2002 . 11991638 . 10.1017/S1355838202021088 . 1370266 . etal.
• Chagnon P, Michaud J, Mitchell G . A missense mutation (R565W) in cirhin (FLJ14728) in North American Indian childhood cirrhosis. . Am. J. Hum. Genet. . 71 . 6 . 1443–9 . 2003 . 12417987 . 10.1086/344580 . 378590 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Obuse C, Yang H, Nozaki N . Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase. . Genes Cells . 9 . 2 . 105–20 . 2004 . 15009096 . 10.1111/j.1365-2443.2004.00705.x . 21813024 . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Ewing RM, Chu P, Elisma F . Large-scale mapping of human protein-protein interactions by mass spectrometry. . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . etal.

Notes and References

1. Zhou Z, Reed R . Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing . EMBO J . 17 . 7 . 2095–106 . Jun 1998 . 9524131 . 1170554 . 10.1093/emboj/17.7.2095 .
2. Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N . Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain . DNA Res . 3 . 5 . 321–9, 341–54 . May 1997 . 9039502 . 10.1093/dnares/3.5.321 . free .
3. Web site: Entrez Gene: DHX38 DEAH (Asp-Glu-Ala-His) box polypeptide 38.