DDX17 explained

Probable ATP-dependent RNA helicase DDX17 (p72) is an enzyme that in humans is encoded by the DDX17 gene.^{[1] [2]}

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species but not by dsDNA. This protein and that encoded by DDX5 gene are more closely related to each other than to any other member of the DEAD box family. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms with the longer transcript reported to also initiate translation at a non-AUG (CUG) start site.

Interactions

DDX17 has been shown to interact with:

• DDX5 (p68),^[3]
• DHX9 (RNA Helicase A),^[4]
• HDAC1,^[5]

Further reading

• Lüking A, Stahl U, Schmidt U . The protein family of RNA helicases . Crit. Rev. Biochem. Mol. Biol. . 33 . 4 . 259–96 . 1998 . 9747670 . 10.1080/10409239891204233 .
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA . A "double adaptor" method for improved shotgun library construction . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 .
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA . Large-Scale Concatenation cDNA Sequencing . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 139146 . 10.1101/gr.7.4.353 .
• Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP . The DNA sequence of human chromosome 22 . Nature . 402 . 6761 . 489–95 . 1999 . 10591208 . 10.1038/990031 . 1999Natur.402..489D . free .
• Uhlmann-Schiffler H, Rössler OG, Stahl H . The mRNA of DEAD box protein p72 is alternatively translated into an 82-kDa RNA helicase . J. Biol. Chem. . 277 . 2 . 1066–75 . 2002 . 11675387 . 10.1074/jbc.M107535200 . free .
• Hönig A, Auboeuf D, Parker MM, O'Malley BW, Berget SM . Regulation of Alternative Splicing by the ATP-Dependent DEAD-Box RNA Helicase p72 . Mol. Cell. Biol. . 22 . 16 . 5698–707 . 2002 . 12138182 . 133985 . 10.1128/MCB.22.16.5698-5707.2002 .
• Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T . A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein . J. Biol. Chem. . 277 . 43 . 40362–7 . 2002 . 12171929 . 10.1074/jbc.M206194200 . free .
• Lee CG . RH70, a bidirectional RNA helicase, co-purifies with U1snRNP . J. Biol. Chem. . 277 . 42 . 39679–83 . 2002 . 12193588 . 10.1074/jbc.C200337200 . free .
• Ogilvie VC, Wilson BJ, Nicol SM, Morrice NA, Saunders LR, Barber GN, Fuller-Pace FV . The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells . Nucleic Acids Res. . 31 . 5 . 1470–80 . 2003 . 12595555 . 149829 . 10.1093/nar/gkg236 .
• Fujita T, Kobayashi Y, Wada O, Tateishi Y, Kitada L, Yamamoto Y, Takashima H, Murayama A, Yano T, Baba T, Kato S, Kawabe Y, Yanagisawa J . Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells . J. Biol. Chem. . 278 . 29 . 26704–14 . 2003 . 12738788 . 10.1074/jbc.M301031200 . free .
• Li J, Hawkins IC, Harvey CD, Jennings JL, Link AJ, Patton JG . Regulation of Alternative Splicing by SRrp86 and Its Interacting Proteins . Mol. Cell. Biol. . 23 . 21 . 7437–47 . 2003 . 14559993 . 207616 . 10.1128/MCB.23.21.7437-7447.2003 .
• Wilson BJ, Bates GJ, Nicol SM, Gregory DJ, Perkins ND, Fuller-Pace FV . The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner . BMC Mol. Biol. . 5 . 11 . 2005 . 15298701 . 514542 . 10.1186/1471-2199-5-11 . free .
• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization . Curr. Biol. . 14 . 16 . 1436–50 . 2004 . 15324660 . 10.1016/j.cub.2004.07.051 . 2371325 . free . 2004CBio...14.1436J .
• Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S . Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions . Genome Res. . 14 . 9 . 1711–8 . 2004 . 15342556 . 515316 . 10.1101/gr.2435604 .
• Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I . A genome annotation-driven approach to cloning the human ORFeome . Genome Biol. . 5 . 10 . R84 . 2005 . 15461802 . 545604 . 10.1186/gb-2004-5-10-r84 . free .

Notes and References

1. Lamm GM, Nicol SM, Fuller-Pace FV, Lamond AI . p72: a human nuclear DEAD box protein highly related to p68 . Nucleic Acids Res . 24 . 19 . 3739–47 . Dec 1996 . 8871553 . 146168 . 10.1093/nar/24.19.3739 .
2. Web site: Entrez Gene: DDX17 DEAD (Asp-Glu-Ala-Asp) box polypeptide 17.
3. Ogilvie VC, Wilson BJ, Nicol SM, Morrice NA, Saunders LR, Barber GN, Fuller-Pace FV . The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells . Nucleic Acids Res. . 31 . 5 . 1470–80 . 12595555 . 149829 . 10.1093/nar/gkg236. 2003 .
4. Wilson BJ, Giguère V . Identification of novel pathway partners of p68 and p72 RNA helicases through Oncomine meta-analysis . BMC Genomics . 8 . 419 . Nov 2007 . 18005418 . 3225811 . 10.1186/1471-2164-8-419 . free .
5. Wilson BJ, Bates GJ, Nicol SM, Gregory DJ, Perkins ND, Fuller-Pace FV . The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner . BMC Mol. Biol. . 5 . 11 . Aug 2004 . 15298701 . 514542 . 10.1186/1471-2199-5-11 . free .