DDX1 explained

ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene.^{[1] [2]}

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

Interactions

DDX1 has been shown to interact with HNRPK.^[3] DDX1 has also been tentatively shown to interact with hCLE/C14orf166/RTRAF and HSPC117 in a cap-binding complex that activates mRNA translation. ^[4]

Further reading

• Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY . Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions . Cell Research . 15 . 11–12 . 923–34 . 2006 . 16354571 . 10.1038/sj.cr.7290370 . free .
• Godbout R, Squire J . Amplification of a DEAD box protein gene in retinoblastoma cell lines . Proceedings of the National Academy of Sciences of the United States of America . 90 . 16 . 7578–82 . August 1993 . 7689221 . 47185 . 10.1073/pnas.90.16.7578 . 1993PNAS...90.7578G . free .
• Kitajima Y, Yatsuki H, Zhang R, Matsuhashi S, Hori K . A novel human homologue of a dead-box RNA helicase family . Biochemical and Biophysical Research Communications . 199 . 2 . 748–54 . March 1994 . 8135819 . 10.1006/bbrc.1994.1292 .
• Akiyama K, Akao Y, Yokoyama M, Nakagawa Y, Noguchi T, Yagi K, Nishi Y . Expression of two dead box genes (DDX1 and DDX6) is independent of that of MYCN in human neuroblastoma cell lines . Biochemistry and Molecular Biology International . 47 . 4 . 563–8 . April 1999 . 10319407 . 10.1080/15216549900201603 . 45609314 . free .
• Golembowski S, Gellrich S, von Zimmermann M, Rutz S, Lippert S, Audring H, Lorenz P, Sterry W, Jahn S . Clonal evolution in a primary cutaneous follicle center B cell lymphoma revealed by single cell analysis in sequential biopsies . Immunobiology . 201 . 5 . 631–44 . April 2000 . 10834319 . 10.1016/s0171-2985(00)80080-7 .
• Bléoo S, Sun X, Hendzel MJ, Rowe JM, Packer M, Godbout R . Association of human DEAD box protein DDX1 with a cleavage stimulation factor involved in 3'-end processing of pre-MRNA . Molecular Biology of the Cell . 12 . 10 . 3046–59 . October 2001 . 11598190 . 60154 . 10.1091/mbc.12.10.3046 .
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI . Directed proteomic analysis of the human nucleolus . Current Biology . 12 . 1 . 1–11 . January 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . 14132033 . free . 2002CBio...12....1A .
• Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ . An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K . The Journal of Biological Chemistry . 277 . 43 . 40403–9 . October 2002 . 12183465 . 10.1074/jbc.M206981200 . 25252628 . free .
• Fang J, Kubota S, Yang B, Zhou N, Zhang H, Godbout R, Pomerantz RJ . A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev . Virology . 330 . 2 . 471–80 . December 2004 . 15567440 . 10.1016/j.virol.2004.09.039 .
• Krishnan V, Zeichner SL . Alterations in the expression of DEAD-box and other RNA binding proteins during HIV-1 replication . Retrovirology . 1 . 42 . December 2004 . 15588285 . 543576 . 10.1186/1742-4690-1-42 . free .
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . January 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Fang J, Acheampong E, Dave R, Wang F, Mukhtar M, Pomerantz RJ . The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytes . Virology . 336 . 2 . 299–307 . June 2005 . 15892970 . 10.1016/j.virol.2005.03.017 .
• Kaneko S, Ohira M, Nakamura Y, Isogai E, Nakagawara A, Kaneko M . Relationship of DDX1 and NAG gene amplification/overexpression to the prognosis of patients with MYCN-amplified neuroblastoma . Journal of Cancer Research and Clinical Oncology . 133 . 3 . 185–92 . March 2007 . 17028906 . 10.1007/s00432-006-0156-y . 9295969 .
• Tu LC, Yan X, Hood L, Lin B . Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells . Molecular & Cellular Proteomics . 6 . 4 . 575–88 . April 2007 . 17220478 . 10.1074/mcp.M600249-MCP200 . 7203032 . free.

Notes and References

1. Schmid SR, Linder P . D-E-A-D protein family of putative RNA helicases . Molecular Microbiology . 6 . 3 . 283–91 . February 1992 . 1552844 . 10.1111/j.1365-2958.1992.tb01470.x . 45727289 . free .
2. Web site: Entrez Gene: DDX1 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1.
3. Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ . An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K . The Journal of Biological Chemistry . 277 . 43 . 40403–9 . October 2002 . 12183465 . 10.1074/jbc.M206981200 . 25252628 . free .
4. Cho WJ, Yoon WJ, Moon CH, Cha SJ, Song H, Cho HR, Jang SJ, Chung DK, Jeong CS, Park JW . Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5 . en . The Journal of Biological Chemistry . 277 . 44 . 41489–96 . November 2002 . 12183466 . 10.1074/jbc.M207104200 . free .