DDOST explained

Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 48 kDa subunit is an enzyme that in humans is encoded by the DDOST gene.^{[1] [2]}

This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Further reading

• Kumar V, Korza G, Heinemann FS, Ozols J . Human oligosaccharyltransferase: isolation, characterization, and the complete amino acid sequence of 50-kDa subunit . Arch. Biochem. Biophys. . 320 . 2 . 217–23 . 1995 . 7625827 . 10.1016/0003-9861(95)90003-9 .
• Nagase T, Miyajima N, Tanaka A . Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1 . DNA Res. . 2 . 1 . 37–43 . 1995 . 7788527 . 10.1093/dnares/2.1.37 . etal. free .
• Stitt AW, He C, Vlassara H . Characterization of the advanced glycation end-product receptor complex in human vascular endothelial cells . Biochem. Biophys. Res. Commun. . 256 . 3 . 549–56 . 1999 . 10080935 . 10.1006/bbrc.1999.0291 .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Lu C, He JC, Cai W . Advanced glycation endproduct (AGE) receptor 1 is a negative regulator of the inflammatory response to AGE in mesangial cells . Proc. Natl. Acad. Sci. U.S.A. . 101 . 32 . 11767–72 . 2004 . 15289604 . 10.1073/pnas.0401588101 . 511050 . 2004PNAS..10111767L . etal. free .
• Suzuki Y, Yamashita R, Shirota M . Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions . Genome Res. . 14 . 9 . 1711–8 . 2004 . 15342556 . 10.1101/gr.2435604 . 515316 . etal.
• Gerhard DS, Wagner L, Feingold EA . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Shibatani T, David LL, McCormack AL . Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits . Biochemistry . 44 . 16 . 5982–92 . 2005 . 15835887 . 10.1021/bi047328f . etal.
• Ewing RM, Chu P, Elisma F . Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . etal.

Notes and References

1. Yamagata T, Tsuru T, Momoi MY, Suwa K, Nozaki Y, Mukasa T, Ohashi H, Fukushima Y, Momoi T . Genome organization of human 48-kDa oligosaccharyltransferase (DDOST) . Genomics . 45 . 3 . 535–40 . Jan 1998 . 9367678 . 10.1006/geno.1997.4966 .
2. Web site: Entrez Gene: DDOST dolichyl-diphosphooligosaccharide-protein glycosyltransferase.