DCLK1 explained

Doublecortin-like kinase protein 1 (DCLK1)^{[1] [2] [3] [4]} is an enzyme that in humans is encoded by the DCLK1 gene.^{[1] [2] [4]} Its C-terminal domain in rats is expressed independently from an alternative transcript, cpg16, and can function alone as a serine/threonine protein kinase that is cyclic AMP dependent.^{[5] [6]}

DCLK1 expression is a marker for tuft cells, a type of chemosensory cell of the intestinal epithelium.^{[3] [7]}

Further reading

• Nagase T, Ishikawa K, Nakajima D . Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. . DNA Res. . 4 . 2 . 141–50 . 1997 . 9205841 . 10.1093/dnares/4.2.141 . etal. free .
• Matsumoto N, Pilz DT, Ledbetter DH . Genomic structure, chromosomal mapping, and expression pattern of human DCAMKL1 (KIAA0369), a homologue of DCX (XLIS). . Genomics . 56 . 2 . 179–83 . 1999 . 10051403 . 10.1006/geno.1998.5673 .
• Burgess HA, Reiner O . Alternative splice variants of doublecortin-like kinase are differentially expressed and have different kinase activities. . J. Biol. Chem. . 277 . 20 . 17696–705 . 2002 . 11884394 . 10.1074/jbc.M111981200 . free .
• Kim MH, Derewenda U, Devedjiev Y . Purification and crystallization of the N-terminal domain from the human doublecortin-like kinase. . Acta Crystallogr. D . 59 . Pt 3 . 502–5 . 2003 . 12595708 . 10.1107/S0907444903000027 . 2003AcCrD..59..502K . etal.
• Kim MH, Cierpicki T, Derewenda U . The DCX-domain tandems of doublecortin and doublecortin-like kinase. . Nat. Struct. Biol. . 10 . 5 . 324–33 . 2003 . 12692530 . 10.1038/nsb918 . 10173864 . etal.
• Seet LF, Liu N, Hanson BJ, Hong W . Endofin recruits TOM1 to endosomes. . J. Biol. Chem. . 279 . 6 . 4670–9 . 2004 . 14613930 . 10.1074/jbc.M311228200 . free .
• Ballif BA, Villén J, Beausoleil SA . Phosphoproteomic analysis of the developing mouse brain. . Mol. Cell. Proteomics . 3 . 11 . 1093–101 . 2005 . 15345747 . 10.1074/mcp.M400085-MCP200 . etal. free .

Notes and References

1. Omori Y, Suzuki M, Ozaki K, Harada Y, Nakamura Y, Takahashi E, Fujiwara T . Expression and chromosomal localization of KIAA0369, a putative kinase structurally related to Doublecortin . J Hum Genet . 43 . 3 . 169–77 . Oct 1998 . 9747029 . 10.1007/s100380050063 . free .
2. Sossey-Alaoui K, Srivastava AK . DCAMKL1, a brain-specific transmembrane protein on 13q12.3 that is similar to doublecortin (DCX) . Genomics . 56 . 1 . 121–6 . May 1999 . 10036192 . 10.1006/geno.1998.5718 .
3. Ding L, Weygant N, Ding C, Lai Y, Li H. DCLK1 and tuft cells: Immune-related functions and implications for cancer immunotherapy. Critical Reviews in Oncology and Hematology. 191. Art. No. 104118. 2023. 10.1016/j.critrevonc.2023.104118. 37660932.
4. Web site: Entrez Gene: DCAMKL1 doublecortin and CaM kinase-like 1. 30 May 2024. 7 April 2024. Gene. National Library of Medicine.
5. Silverman MA, Benard O, Jaaro H, Rattner A, Citri Y, Seger R. CPG16, a novel protein serine/threonine kinase downstream of cAMP-dependent protein kinase. Journal of Biological Chemistry. 1999. 274. 5. 2631-2636. free. 9915791. 10.1074/jbc.274.5.2631.
6. Lin PT, Gleeson JG, Corbo JC . DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization. . J. Neurosci. . 20 . 24 . 9152–61 . 2001 . 11124993 . 10.1523/JNEUROSCI.20-24-09152.2000. 6773030 . etal. free .
7. Gerbe F, Legraverend C, Jay P. The intestinal epithelium tuft cells: specification and function. Cellular and Molecular Life Sciences. 69. 2907-2917. 2012. 17. 22527717. 3417095. 10.1007/s00018-012-0984-7. free.