DAD1 explained
Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit DAD1 is an enzyme that in humans is encoded by the DAD1 gene.[1]
Function
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.
Interactions
DAD1 has been shown to interact with MCL1.[2]
References
Further reading
- Yulug IG, See CG, Fisher EM, Ylug IG . The DAD1 protein, whose defect causes apoptotic cell death, maps to human chromosome 14 . Genomics . 26 . 2 . 433–5 . 1995 . 7601483 . 10.1016/0888-7543(95)80241-D .
- Apte SS, Mattei MG, Seldin MF, Olsen BR . The highly conserved defender against the death 1 (DAD1) gene maps to human chromosome 14q11-q12 and mouse chromosome 14 and has plant and nematode homologs . FEBS Lett. . 363 . 3 . 304–6 . 1995 . 7737422 . 10.1016/0014-5793(95)00321-Y . 39270530 .
- Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
- Nakashima T, Sekiguchi T, Kuraoka A, Fukushima K, Shibata Y, Komiyama S, Nishimoto T . Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells . Mol. Cell. Biol. . 13 . 10 . 6367–74 . 1993 . 8413235 . 364695 . 10.1128/mcb.13.10.6367.
- Kelleher DJ, Gilmore R . DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase . Proc. Natl. Acad. Sci. U.S.A. . 94 . 10 . 4994–9 . 1997 . 9144178 . 24619 . 10.1073/pnas.94.10.4994 . 1997PNAS...94.4994K . free .
- Makishima T, Nakashima T, Nagata-Kuno K, Fukushima K, Iida H, Sakaguchi M, Ikehara Y, Komiyama S, Nishimoto T . The highly conserved DAD1 protein involved in apoptosis is required for N-linked glycosylation . Genes Cells . 2 . 2 . 129–41 . 1997 . 9167970 . 10.1046/j.1365-2443.1997.1070303.x . 25807010 . free .
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
- Zhong XP, Krangel MS . Enhancer-blocking activity within the DNase I hypersensitive site 2 to 6 region between the TCR alpha and Dad1 genes . J. Immunol. . 163 . 1 . 295–300 . 1999 . 10.4049/jimmunol.163.1.295 . 10384128 . free .
- Makishima T, Yoshimi M, Komiyama S, Hara N, Nishimoto T . A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family . J. Biochem. . 128 . 3 . 399–405 . 2000 . 10965038 . 10.1093/oxfordjournals.jbchem.a022767 .
- Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J . Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides . Nat. Biotechnol. . 21 . 5 . 566–9 . 2003 . 12665801 . 10.1038/nbt810 . 23783563 .
- Shibatani T, David LL, McCormack AL, Frueh K, Skach WR . Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits . Biochemistry . 44 . 16 . 5982–92 . 2005 . 15835887 . 10.1021/bi047328f .
Notes and References
- Web site: Entrez Gene: DAD1 defender against cell death 1.
- Makishima T, Yoshimi M, Komiyama S, Hara N, Nishimoto T . A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family . J. Biochem. . 128 . 3 . 399–405 . September 2000 . 10965038 . 10.1093/oxfordjournals.jbchem.a022767 .
