D-Ala-D-Ala dipeptidase explained

D-Ala-D-Ala dipeptidase
Ec Number:	3.4.13.22

D-Ala-D-Ala dipeptidase (D-alanyl-D-alanine dipeptidase, vanX D-Ala-D-Ala dipeptidase, VanX) is an enzyme.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

D-Ala-D-Ala + H2O

\rightleftharpoons 2 D-Ala

This enzyme is Zn²⁺-dependent.

Notes and References

1. Reynolds PE, Depardieu F, Dutka-Malen S, Arthur M, Courvalin P . Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine . Molecular Microbiology . 13 . 6 . 1065–70 . September 1994 . 7854121 . 10.1111/j.1365-2958.1994.tb00497.x .
2. Wu Z, Wright GD, Walsh CT . Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147 . Biochemistry . 34 . 8 . 2455–63 . February 1995 . 7873524 . 10.1021/bi00008a008 .
3. McCafferty DG, Lessard IA, Walsh CT . Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX . Biochemistry . 36 . 34 . 10498–505 . August 1997 . 9265630 . 10.1021/bi970543u .
4. Bussiere DE, Pratt SD, Katz L, Severin JM, Holzman T, Park CH . The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance . Molecular Cell . 2 . 1 . 75–84 . July 1998 . 9702193 . 10.1016/s1097-2765(00)80115-x . free .
5. Tan AL, Loke P, Sim TS . Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2) . Research in Microbiology . 153 . 1 . 27–32 . 2002 . 11881895 . 10.1016/s0923-2508(01)01282-7 . free .
6. Matthews ML, Periyannan G, Hajdin C, Sidgel TK, Bennett B, Crowder MW . Probing the reaction mechanism of the D-ala-D-ala dipeptidase, VanX, by using stopped-flow kinetic and rapid-freeze quench EPR studies on the Co(II)-substituted enzyme . Journal of the American Chemical Society . 128 . 40 . 13050–1 . October 2006 . 17017774 . 2547881 . 10.1021/ja0627343 .