Cystocentesis is a veterinary procedure where a needle is placed into the urinary bladder through the abdominal wall of an animal and a sample of urine is removed.[1] Diagnostic cystocentesis is used to prevent the sample taken for urinalysis from being contaminated with bacteria, cells and debris from the lower urogenital tract.[2] Therapeutic cystocentesis may be employed to relieve pressure buildup due to urethral obstruction. Therapeutic cystocentesis is typically last resort for relief, as a greatly distended bladder is more likely to rupture when punctured. A safer method of emptying the urinary bladder in this condition is by urethral catheterization.