Cypridina-luciferin 2-monooxygenase explained

Cypridina-luciferin 2-monooxygenase
Ec Number:	1.13.12.6
Cas Number:	61969-99-1
Go Code:	0047712

In enzymology, a Cypridina-luciferin 2-monooxygenase is an enzyme that catalyzes the chemical reaction

Cypridina luciferin + O₂

\rightleftharpoons oxidized Cypridina luciferin + CO₂ + hnu

Thus, the two substrates of this enzyme are Cypridina luciferin and O₂, whereas its 3 products are oxidized Cypridina luciferin, CO₂, and light.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O with incorporation of one atom of oxygen (internal monooxygenases o internal mixed-function oxidases). The systematic name of this enzyme class is Cypridina-luciferin:oxygen 2-oxidoreductase (decarboxylating). Other names in common use include Cypridina-type luciferase, luciferase (Cypridina luciferin), and Cypridina luciferase.

The primary sequence was determined by cloning the cDNA.^[1]

References

• Cormier MJ, ((Crane JM Jr)), Nakano Y . 1967 . Evidence for the identity of the luminescent systems of Porichthys porosissimus (fish) and Cypridina hilgendorfii (crustacean) . Biochem. Biophys. Res. Commun. . 29 . 747 - 52 . 5624784 . 10.1016/0006-291X(67)90281-1 . 5 .
• Karpetsky TP, White EH . 1973 . The synthesis of Cypridina etioluciferamine and the proof of the structure of Cypridina luciferin . Tetrahedron . 29 . 3761 - 3773 . 10.1016/0040-4020(73)80193-0 . 23 .
• Kishi Y, Goto T, Hirata Y, Shiromura O, Johnson FH . Kishi Y . 1966 . Cypridina bioluminescence. I. Structure of Cypridina luciferin . Tetrahedron Lett. . 7 . 10.1016/S0040-4039(01)82806-9 . 3427 - 3436 .
• Tsuji FI, ((Lynch RV III)), Stevens CL . 1974 . Some properties of luciferase from the bioluminescent crustacean, Cypridina hilgendorfii . Biochemistry . 13 . 5204 - 9 . 4433517 . 10.1021/bi00722a024 . 25 .

Notes and References

1. Thompson EM, Nagata S, Tsuji FI . Cloning and expression of cDNA for the luciferase from the marine ostracod Vargula hilgendorfii . . 86 . 17 . 6567–71 . 1989 . 2771943 . 297885 . 10.1073/pnas.86.17.6567. free .