| Event: | Cycling, Women's 500m time trial C4-5 |
| Games: | 2012 Summer |
| Venue: | ExCeL Exhibition Centre |
| Dates: | September 1 |
| Competitors: | 14 |
| Nations: | 10 |
| Win Value: | 36.997 |
| Gold: | Sarah Storey |
| Goldnpc: | GBR |
| Silver: | Jennifer Schuble |
| Silvernpc: | USA |
| Bronze: | Ruan Jianping |
| Bronzenpc: | CHN |
The Women's 500m time trial, Classes 1-3 track cycling event at the 2012 Summer Paralympics took place on September 1 at London Velopark.
WR = World Record
| width=20 | Rank! | width=180 | Name ! | Country | Class | Factored Time |
|---|---|---|---|---|---|---|
| Sarah Storey | C5 | 36.997 | ||||
| Jennifer Schuble | C5 | 37.941 | ||||
| Ruan Jianping | C4 | 38.194 WR | ||||
| Anna Harkowska | C5 | 39.599 | ||||
| Greta Neimanas | C5 | 39.621 | ||||
| Susan Powell | C4 | 39.702 | ||||
| Fiona Southorn | C5 | 41.796 | ||||
| Alexandra Green | C4 | 42.095 | ||||
| Megan Fisher | C4 | 42.178 | ||||
| Marie-Claude Molnar | C4 | 42.228 | ||||
| Roxanne Burns | C4 | 42.621 | ||||
| Kerstin Brachtendorf | C5 | 43.186 | ||||
| Annina Schillig | C5 | 43.248 | ||||
| Sara Tretola | C5 | 44.122 |