The women's time trial was held on 21 March on a 29 km course on the St Kilda Foreshore and Beach Road.
| Rank | Rider | Time | |
|---|---|---|---|
| 37:40.87 | |||
| 37:56.07 | |||
| 38:00.32 | |||
| 4 | 39:02.53 | ||
| 5 | 39:26.37 | ||
| 6 | 39:46.02 | ||
| 7 | 40:17.25 | ||
| 8 | 40:32.60 | ||
| 9 | 40:46.17 | ||
| 10 | 41:39.79 | ||
| 11 | 42:58.67 | ||
| 12 | 43:10.73 | ||
| 13 | 43:25.44 | ||
| 14 | 45:59.26 | ||
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