The Cycling at the 1965 SEAP Games was held between 15 September to 18 December. Cycling track were held at the Merdeka Stadium, Kuala Lumpur.
| 1000 m Individual road trial |  Tan Thol | 1:14.4 |  Ng Joo Pong | 1:17.0 |  Pairote Roongtonkit | 1:18.3 | |
| 100 km road team trial | Thailand Pairote Roongtonkit Smaisuk Krisansuwan Chainarong Sophonpong C. Varayout | 2:51:15 | Cambodia Ret Chhon Yi Yuong Van Son Van Ung | 2:53:18 | Trần Văn Nen Tranh Gia Chau Bùi Văn Hoàng Van Dam | 2:53:34 | |
| 800 m Individual massed | Shaharuddin Jaafar | 1:05.8 | Nguyễn Văn Châu | 1:05.8 | Smaisuk Krisansuwan | 1:06.2 | |
| 1600 m Individual massed | Smaisuk Krisansuwan | 2:35.7 | Shaharuddin Jaafar | 2:35.7 | Pairote Roongtonkit | 2:35.8 | |
| 4800 m Individual massed | Yi Yuong | 7:41.8 | Tranh Gia Chau | 7:42.8 | Tan Thol | 7:42.9 | |
| 10,000 m Individual massed | Dechapol | 15:41.6 | Ng Joo Pong | 15:41.7 | Danh Tanh Se | 15:42.2 | |
| 200 km Road massed individual | Trần Văn Nen | 6:10:51.7 | Choy Mow Thim | 6:10:52.2 | Pairote Roongtonkit | 6:10:53.5 | |
| 4000 m Team pursuit | Thailand | Cambodia | | ||||
| Team Placing | Thailand Pairote Roongtonkit Chainarong Sophonpong Surivong | 18:34:45.8 | | 18:34:46.9 | Malaysia | 18:54:04.3 |