Crotonyl-CoA carboxylase/reductase explained
| Crotonyl-CoA carboxylase/reductase |
| Ec Number: | 1.3.1.85 |
| Width: | 270 |
Crotonyl-CoA carboxylase/reductase (CCR, crotonyl-CoA reductase (carboxylating)) is an enzyme with systematic name (2S)-ethylmalonyl-CoA:NADP+ oxidoreductase (decarboxylating).[1] [2] This enzyme catalyses the following chemical reaction
(2S)-ethylmalonyl-CoA + NADP+
(E)-but-2-enoyl-CoA + CO
2 + NADPH + H
+The reaction is catalysed in the reverse direction. This reaction is a part of the ethylmalonyl-CoA pathway (EMC).
Normally, the Glyoxylate cycle is present in microorganisms to assimilate acetate. However, in microorganisms that do not have a Glyoxylate cycle, CCR is important for its role in an alternative pathway to assimilate acetate.
There are various CCR homologues and in S. tsukubaensis' genome the CCR homologues ccr1 and allR were identified and recognized to be a part of two separate metabolic pathways.[3] The homologue ccr1 is involved in the EMC pathway, whereas the homologue allR is involved in biosynthesis of immunosuppressants FK506/FK520.
Notes and References
- Erb TJ, Berg IA, Brecht V, Müller M, Fuchs G, Alber BE . Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway . Proceedings of the National Academy of Sciences of the United States of America . 104 . 25 . 10631–6 . June 2007 . 17548827 . 1965564 . 10.1073/pnas.0702791104 . 2007PNAS..10410631E . free .
- Erb TJ, Brecht V, Fuchs G, Müller M, Alber BE . Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase . Proceedings of the National Academy of Sciences of the United States of America . 106 . 22 . 8871–6 . June 2009 . 19458256 . 2689996 . 10.1073/pnas.0903939106 . 2009PNAS..106.8871E . free .
- Blažič, M., Kosec, G., Baebler, Š., Gruden, K., & Petković, H. (2015). Roles of the crotonyl-CoA carboxylase/reductase homologues in acetate assimilation and biosynthesis of immunosuppressant FK506 in Streptomyces tsukubaensis. Microbial cell factories, 14, 164.
