| Church of the Holy Spirit | |
| Location: | Lake Wales, Florida |
| Coordinates: | 27.8939°N -81.565°W |
| Built: | 1927 |
| Architect: | P.C. Samuell; R.W. Burrows |
| Architecture: | Mission/Spanish Revival |
| Added: | August 31, 1990 |
| Mpsub: | Lake Wales MPS |
| Refnum: | 90001271 |
The former Church of the Holy Spirit is an historic Roman Catholic church building located at 1099 Hesperides Road in Lake Wales, Florida. In 1989 it was sold by the local Catholic diocese to the City of Lake Wales. It is now the Lake Wales Cultural Center.
On August 31, 1990, it was added to the U.S. National Register of Historic Places.