CHU 13 medium is a culture medium used in microbiology for the growth of certain algal species, first published by S.P. Chu in 1942.[1] It is used as growth medium for the biofuel candidate alga Botryococcus braunii.[2] CHU 13 includes essential minerals and trace elements that are required by algae for growth, but does not include a carbon source and so is only appropriate for growth of phototrophs. It can be prepared as either a liquid medium or as an agar medium.
400 | ||
K2HPO4 | 80 | |
107 | ||
200 | ||
20 | ||
100 | ||
0.02 | ||
5.72 | ||
3.62 | ||
ZnSO4 heptahydrate | 0.44 | |
0.16 | ||
0.084 | ||
1 drop |
The remaining volume is pure, de-ionized water.
Because it is difficult to weigh out some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized) water. Correct pH to 7.5, and then autoclave.